两个罗氏沼虾种群的遗传多样性研究

Genetic diversity of two giant freshwater prawn Macrobrachium rosenbergii populations

为了解罗氏沼虾广东种群和广西种群的遗传多样性,指导罗氏沼虾的遗传育种工作,利用12个微卫星标记对罗氏沼虾广西和广东2个种群的遗传多样性进行了分析。结果表明,12个微卫星位点在2个罗氏沼虾种群中的扩增产物均具有多态性,共获得71个等位基因,每个位点平均等位基因数为5.92个。广西种群的平均有效等位基因数、观测杂合度、期望杂合度、平均多态信息含量分别为3.9183、0.9505、0.7376、0.6558,广东种群的平均有效等位基因数、观测杂合度、期望杂合度、平均多态信息含量分别为4.4374、0.9413、0.7609、0.6834,高于广西种群。结果表明,两个罗氏沼虾种群均具有较高的遗传多样性,具有较大的育种潜力。

Twelve microsatellite DNA markers were used to assess the genetic diversity of giant freshwater prawn Macrobrachium rosenbergii of Guangxi population and Guangdong population and to guide the selective breeding work. The results showed that,12 microsatellite loci in the amplification products of two M. rosenbergii groups were all polymorphic,a total of 71 alleles were amplified,and the average number of alleles per locus was 5.92. The average effective number of alleles,observed heterozygosity,expected heterozygosity,and polymorphism information content of Guangxi population were 3.9183,0.9505,0.7376,0.6558,respectively,while those indexes of Guangdong population were 4.4374,0.9413,0.7609,0.6834,respectively,which had higher genetic diversity than Guangxi population. Results demonstrated that the two populations of M. rosenbergii both had high genetic diversity,which had great potential for the selective breeding of M. rosenbergii.