原儿茶酸对帕金森模型大鼠血清TNF-α、IL-1β及氧化应激产物水平的影响

Effect of protocatechuic acid on serum TNF-α,IL-1β and oxidative stress products levels in Parkinson rats

目的探讨原儿茶酸对帕金森模型大鼠血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素1β(interleukin-1β,IL-1β)及氧化应激产物水平的影响。方法选取雄性SD大鼠60只,随机分为正常对照组(n=10)、模型组(n=10)、美多芭组(n=20)和原儿茶酸组(n=20)。模型组、美多芭组和原儿茶酸组建立帕金森大鼠模型,美多芭组和原儿茶酸组大鼠分别给予相应药物治疗,持续2周。治疗结束后,对所有大鼠的血清TNF-α、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)及IL-1β水平进行检测。结果与正常对照组比较,模型组血清TNF-α、IL-1β、MDA水平明显升高,而SOD水平明显降低(P<0.05)。与模型组比较,经治疗后美多芭组和原儿茶酸组血清TNF-α、IL-1β、MDA水平明显降低,而SOD水平明显升高(P<0.05)。美多芭组与原儿茶酸组血清TNF-α、IL-1β、MDA及SOD比较差异无统计学意义。结论原儿茶酸能够明显降低帕金森模型大鼠血清TNF-α、MDA及IL-1β水平,提高SOD活性,对帕金森病引起的氧化应激损伤有保护作用。

Objective To explore the effect of protocatechuic acid on serum tumor necrosis factor-α（ TNF-α）,interleukin-1β（ IL-1β） and oxidative stress products levels in Parkinson rats. Methods 60 male SD rats were randomly divided into normal control group（ n = 10）,model group（ n = 10）,madopar group（ n = 20） and protocatechuic acid group（ n = 20）. Rat model with Parkinson disease were builded in model group,madopar group and protocatechuic acid group. Madopar group and protocatechuic acid group were given corresponding drug with a consecutive treatment of two weeks. After treatment,the serum TNF-α,malondialdehyde（ MDA）,superoxide dismutase（ SOD） and IL-1β levels were detected in all groups. Results Compared with normal control group,the serum TNF-α,IL-1β and MDA levels in model group were significantly higher,and SOD level was lower（ P〈0. 05）. Compared with model group,the serum TNF-α,IL-1β and MDA levels in madopar group pre-treatment were significantly lower,and SOD level was higher（ P〈0. 05）. There were no significant difference of serum TNF-α,IL-1β,MDA and SOD levels between madopar group and protocatechuic acid group. Conclusion The protocatechuic acid could significantly reduce the serum TNF-α,MDA and IL-1β levels in Parkinson model rats,enhance the activity of SOD,which has protective effect on oxidative stress injury induced by Parkinson disease.