高效液相色谱法测定牛黄清火丸中蒽醌类化合物的含量

Determination of anthraquinones in Niuhuang Qinghuo Pills by HPLC

目的建立同时测定牛黄清火丸中大黄酸、大黄素、大黄酚、大黄素甲醚4种蒽醌类化合物含量的方法。方法用20%硫酸水解样品,以丙酮为溶剂,索氏提取法提取样品中的蒽醌类化合物,采用高效液相色谱法对其进行测定,色谱柱为Kromasil C18柱,流动相为甲醇-0.5%磷酸水溶液（75∶25,V/V）,流速为1.0 m L/min,检测波长为230 nm。结果样品中4种蒽醌类化合物分别在1.50~150、0.950~95.0、1.30~130、1.15~115 mg/L范围内呈良好的线性关系,4种化合物加标回收率在98.14%~101.3%之间。结论该方法操作便捷,准确,稳定性好,可用于牛黄清火丸的质量控制。

Objective To establish the method of determination for rhein,emodin,chrysophanol and physcion in Niuhuang Qinghuo Pills.Methods Sample was hydrolyzed by 20% H2SO4,and anthraquinones were extracted by soxhlet extraction with acetone as solvent,and were determined by HPLC with Kromasil C18 as column,methanol- 0. 5% H3PO4（ 75： 25,V/V） as mobile phase at the flow of 1. 0 m L/min,230 nm as the detection wavelength. Results The linear relationship of rhein,emodin,chrysophanol and physcion were 1. 50-150,0. 950-95. 0,1. 30-130 and 1. 15-115 mg / L.The anthraquinones were seperated completely,the recovery of 4 anthraquinones were 98. 14%- 101. 3%. Conclusion This method is simple,accurate,steady,and could be used for the quality control of Niuhuang Qinghuo Pills.