摘要
目的:观察TCDCA对大鼠肺泡巨噬细胞(NR8383)细胞钙调素(CaM)mRNA表达的影响,探讨TCDCA对大鼠肺泡巨噬细胞的作用机制。方法:采用荧光定量PCR技术检测TCDCA对大鼠肺泡巨噬细胞CaMmRNA表达的影响。结果:与空白对照组相比较,高剂量(1×10^(-4)mol/L)、中剂量(1×10^(-5)mol/L)及低剂量(1×10^(-6)mol/L)的TCDCA作用组均能够极显著(P<0.01)的促进大鼠肺泡巨噬细胞CaMmRNA的表达量。结论:TCDCA能够促进大鼠肺泡巨噬细胞CaMmRNA的表达。
Objective Thestudy was aimed to observe effect of taurochenodeoxy-cholic acid（ TCDCA） on calmodulin（ Ca M） expression and investigate the mechanism that the regulation of TCDCA on the rat alveolar macrophages NR8383. Method mRNA expression of Ca M was detected by real-time RT-PCR in rat alveolar macrophages NR8383. Result The resultsshow： Compared with the blank control,TCDCA（ 1 × 10^（-4） mol / L,1 × 10^（-5）mol / L and 1 × 10^（-6）mol / L） markedly promoted the mRNA and protein expression levels of Ca M in rat alveolar macrophages NR8383. Conclusion The main conclusions of thisstudy are as follows： TCDCA can adjust the mRNA expression levels of Ca M in rat alveolar macrophages NR8383.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2015年第5期1-4,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然科学基金资助(31272605)
