青天葵及其近缘种毛叶青天葵的HPLC指纹图谱鉴别研究

HPLC Fingerprints of Nerviliae fordii and Nervilia plicata

目的:建立青天葵HPLC指纹图谱分析方法,并与外形极相似的毛叶青天葵进行比较,为该药材的鉴别及质量评价提供依据。方法:采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.2%磷酸系统为流动相,梯度洗脱;流速为1.0 m L/min;检测波长为256 nm;柱温为室温。测定了15批青天葵以及5批毛叶青天葵的HPLC图谱。采用中国药典推荐的"中药色谱指纹图谱相似度评价系统"(2004 A版)对HPLC图谱进行分析。结果:青天葵与毛叶青天葵的HPLC指纹图谱差异显著;大叶青天葵与小叶青天葵的HPLC指纹图谱无显著差异。结论:所建立的HPLC指纹图谱方法可以用于青天葵药材的质量评价,并能有效地将青天葵与其代用品毛叶青天葵区分开来。

Objective： To establish the HPLC fingerprint of the whole plant of Nerviliae fordii and Nervilia plicata,in order to provide a method for evaluation of authenticity and quality control of the whole plant of Nerviliae fordii. Methods： 15 batches of Nerviliae fordii and 5 batches of Nervilia plicata were analyzed on a Kromasil C18column（ 250 mm × 4. 6 mm,5 μm） at room temperature with gradient elution using acetonitrile and 0. 2% phosphoric acid as the mobile phase at a flow rate of 1. 0 m L / min. The wavelength of detector was 256 nm. Results： Significant differences were found between Nerviliae fordii and Nervilia plicata. Furthermore,there were no obvious differences observed between Nerviliae fordii of big-leaf and Nerviliae fordii of small-leaf. Conclusion： The present-developed HPLC fingerprints method provides a rapid,effective and valuable benchmark for distinguishing of Nerviliae fordii and Nervilia plicata,which is favorable to improve overall quality control of Nerviliae Fordii.