荨麻多糖对D-半乳糖致衰老小鼠的抗衰老作用

Anti-aging Effect of Urtica Polysaccharides in D-galactose Induced Aging Mice

目的:研究荨麻多糖对D-半乳糖致衰老模型小鼠的抗衰老作用。方法:将90只C57BL小鼠随机分为正常对照组、模型组、VE(100 mg/kg)阳性对照组及荨麻多糖高(200 mg/kg)、中(100 mg/kg)、低(50 mg/kg)剂量组6组,正常对照组每日皮下注射生理盐水(10 m L/kg),其余各组小鼠均每日皮下注射D-半乳糖(150 mg/kg)造模,同时6组小鼠分别灌胃相应药物,正常对照组与模型组灌胃等体积生理盐水。给药42 d后,称量体质量,进行八臂迷宫试验和游泳耐力测验。随后处死各组小鼠,采集小鼠的血清、肝、脑检测其中总蛋白含量,并用试剂盒检测肝、脑总抗氧化能力(T-AOC),总超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果:与模型组比较,荨麻多糖组小鼠体质量增长幅度较大,游泳耐力更好,迷宫通过时间缩短,错误次数减少,血清与肝、脑组织蛋白含量及肝、脑组织总抗氧化能力和SOD、GSH-Px活性显著升高,而肝、脑组织MDA含量显著降低。结论:荨麻多糖对D-半乳糖致衰老小鼠有一定的抗衰老作用,其机制可能与抗氧化作用有关。

Objective： To investigate the anti-aging effect of polysaccharides from Urtica lobatifolia（ Urtica polysaccharides） on subacute aging mice induced by D-galactose. Methods： 90 mice were randomly divided into six groups： normal group,aging mice model group,VEgroup[100 mg /（ kg·d）,ig],high level of Urtica polysaccharides group[200 mg /（ kg·d）,ig],medium level of Urtica polysaccharides group[100 mg /（ kg·d）,ig]and low level of Urtica polysaccharides group[50 mg /（ kg·d）,ig]. The normal group was injected saline[10 m L /（ kg·d）,sc],while the other groups were injected D-galactose[150 mg /（ kg·d）,sc]. After six weeks,all the animals were weighed. After eight arm maze experiment and swimming endurance experiment,serum,liver and brain was collected. The content of protein in serum,liver and brain was detected. Total antioxidant capacity（ T-AOC）,activities of total superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-Px）,and malondialdehyde（ MDA） content in liver and brain samples were evaluated by kits. Results： Compared with the model group,Urtica polysaccharides groups mice had larger body weight,longer swimming time,shorter time out of the maze and fewer numbers of error,as well as higher protein content in serum,liver and brain. The capacity of T-AOC,the activities of SOD and GSH-Px of polysaccharides groups in brain and liver tissue were increased significantly,and the MDA content was decreased significantly. Conclusion： Polysaccharides from Urtica lobatifolia has anti-aging effects on aging model mice,and the mechanism may be related to its antioxidant effect.