系统性红斑狼疮患者的白细胞介素17阳性调节性T细胞对B细胞的存活及抗体分泌的调节

The modulation of B cells survival and secretion of antibodies by interleukin-17-producing T cells in patients with systemic lupus erythematosus

目的:采用体外实验观察系统性红斑狼疮(systemic lupus erythematosus,SLE)患者分泌白细胞介素17(interleukin,IL-17)的调节性T细胞在促进B细胞的存活及抗体分泌中的作用。方法 :采用流式细胞仪分选20例活动期SLE患者及20名健康对照者外周血调节性T细胞的3个功能亚群和B细胞,并分组、分条件共培养,分时段进行细胞功能实验。结果:在活动期SLE患者中,CD45RA-Foxp3low CD25+非抑制性调节性T细胞亚群分泌IL-17明显增加,能够增强CD19+B细胞存活并促进其抗体产生;而加入抗IL-17抗体阻断后,该群细胞的正向促进功能减退(P<0.05)。结论 :SLE患者中增多的不具抑制功能的CD45RA-Foxp3low CD25+调节性T细胞,可能通过分泌大量的IL-17,促进B细胞的存活、增殖,并向浆细胞分化,分泌大量的自身抗体,参与疾病的发生、发展。

Objective： To study the interleukin-17（IL-17）-producing-Tregs for promoting the survival and antibodies secretion of B cells in systemic lupus erythematosus（SLE） in vitro. Methods： Twenty patients with active SLE were enrolled and 20 heathy volunteers were served as controls. Blood mononuclear cells were sort out for the three functional subpopulations of regulatory T cells and B cells by flow cytometry. The obtained cells were grouped and co-cultured under different conditions. Results： CD45RA-Fox P3^lowCD25^＋non-Tregs subpopulation secreted more IL-17 in SLE, which promoted B cells survival and the secretion of antibodies. These promotion fuctions were attenuated when anti IL-17 was added（P〈0.05）. Conclusions： CD45RA-Fox P3^lowCD25^＋non-Tregs subpopulation is increased in SLE patients and could promote B cells survival and the secretion of antibodies via prodcuing a great deal of IL-17, which might facilitate the development of the disease.