抗核抗体谱检测在系统性红斑狼疮诊断中的临床价值

The clinical value of antinuclear antibody spectrum for diagnosing systemic lupus erythematosus

目的 :研究抗核抗体谱(antinuclear antibody spectrum,ANAs)检测在系统性红斑狼疮(systemic lupus erythematosus,SLE)诊断中的应用价值。方法:采用线性免疫分析法检测58例SLE患者(SLE组)、53例其他自身免疫性疾病(疾病对照组)和60名健康体检者(正常对照组)血清样本中的ANAs共计8项抗体,比较分析3组间的8项抗体阳性检出率及在自身免疫病中单项抗体检测与多项抗体联合检测的阳性检出率。结果:SLE组ANAs 8项抗体中5项抗体的阳性检出率明显高于疾病对照组及正常对照组,差异有统计学意义(P<0.05);而疾病对照组ANAs 8项抗体中6项抗体的阳性检出率虽略有高于正常对照组的趋势,但差异尚无统计学意义(P>0.05)。SLE组单项抗体检测与多项抗体联合检测的阳性检出率比较,差异有统计学意义(P<0.05);而疾病对照组中单项抗体检测与多项抗体联合检测的阳性检出率比较,差异无统计学意义(P>0.05)。结论:ANAs的8项抗体检测有助于自身免疫性疾病的诊断、病情判断和治疗,多项抗体联合检测为SLE的筛查、分类等方面提供了重要的实验室依据,具有一定的临床应用价值。

Objective： To explore the clinical value of antinuclear antibody spectrum（ANAs）detection for the diagnosis of systemic lupus erythematosus（SLE）. Methods： Fifty-eight SLE patients were enrolled, and 53 patients with other autoimmune disease and 60 healthy volunteers were served as the control groups. Eight types of ANAs in serum were detected by linear immunoassay（LIA）. Positive rates of these types of antibodies were compared between the three groups.Results： The positive rates of 5 types of ANAs were higher in SLE groups than those in the other two groups（P〈0.05）. The positive rates of 6 types of ANAs were slightly higher in autoimmune disease control group than in healthy control group,but were not statistically significant（P〉0.05）. In SLE group, the positive rate of multiple antibody detection was higher than that of single antibody detection（P〉0.05）, while no such differences were seen in the other two control groups（P〈0.05）.Conclusions： Eight types of ANAs detection is useful for the diagnosis, severity assessment and treatment of autoimmune disease. Multiple types of ANAs detection could provide evidences for SLE screening and SLE classification.