氟对小鼠肠道优势菌生长情况及其糖代谢酶活性的影响

EFFECTS OF FLUORIDE ON THE GROWTH CHARACTERISTICS AND METABOLIC ENZYMES ACTIVITY OF THE PREDOMINANT INTESTINAL BACTERIA IN MICE INTESTINE

试验选用6周龄左右的健康小鼠,取回盲肠部黏液进行大肠杆菌和乳酸杆菌的分离培养,在培养基中添加不同浓度（0、0.1、1、10、100mmol/L）的氟化钠（NaF）。分别测定不同浓度氟对大肠杆菌和乳酸杆菌的生长情况及其己糖激酶（HK）、丙酮酸激酶（PK）、琥珀酸脱氢酶（SDH）、柠檬酸合酶（CS）和乳酸脱氢酶（LDH）的代谢酶活性。结果显示,0.1 mmol/L和1mmol/L浓度的Na F在作用0-12h内,对大肠杆菌和乳酸杆菌菌液的生长OD变化值均有显著促进作用,而高浓度（10mmol/L和100 mmol/L）氟则显著抑制大肠杆菌和乳酸杆菌的生长OD变化值,但随时间推移,促进和抑制作用均减弱。此外,氟能够抑制大肠杆菌和乳酸杆菌糖代谢过程的HK、PK、SDH、CS和LDH的酶活性。由此推断,低浓度氟对大肠杆菌和乳酸杆菌的生长有促进作用,高浓度氟则抑制大肠杆菌和乳酸杆菌的生长。而且,氟对大肠杆菌和乳酸杆菌糖代谢过程中的HK、PK、SDH、CS和LDH的酶活性有抑制作用。

This study was conducted to evaluate the effect of Na F on growth and glycolytic metabolic enzymes of E. coli and Lactobacillus in mice intestine. Six- week- old healthy mice were selected to retrieve the mucus of the appendix and E. coli and Lactobacillus were isolated and cultured. Different concentrations with 0,0. 1,1,10 and 100 mmol / L sodium fluoride（ Na F） were added to the culture medium. E. coli and Lactobacillus growth and hexokinase（ HK）,pyruvate kinase（ PK）,succinate dehydrogenase（ SDH） and citric acid synthase（ CS） and lactate dehydrogenase（ LDH） metabolic enzyme activity were all detected. The results showed that,the growth of E. coli and Lactobacillus were significantly promoted by 0. 1 mmol / Land1 mmol / L NaF from 0 - 12 h,while the high concentration（ 10 mmol / L and 100 mmol / L） significantly inhibited the growth of E. coli and Lactobacillus. However,the promotion and inhibition were attenuated with time extension. In addition,fluoride（ F） inhibited the activity of HK,PK,SDH,CS and LDH in the process of glucose metabolism of E. coli and Lactobacillus. In summary,low concentration of fluoride promoted the growth of E. coli and Lactobacillus,and the growth of E. coli and Lactobacillus were inhibited by high concentration fluoride. Moreover,there was an inhibition of fluoride to the activity of HK,PK,SDH,CS and LDH in the glucose metabolism of E. coli and Lactobacillus.