围生期毒死蜱暴露致子代8周雄鼠睾丸组织的氧化损伤

Perinatal Exposure to Chlorpyrifos Induced Testicular Oxidative Damage in 8 Week Offspring Male Rat

毒死蜱是目前全世界使用和销售量最大的有机磷杀虫剂之一。为探讨围生期毒死蜱暴露致8周雄性子鼠睾丸组织的氧化损伤,选择健康Wistar妊娠母鼠于妊娠期(gestation days,GD)第6天至子鼠出生后(postnatal days,PND)21天通过灌胃染毒0、0.75、1.35和2.70 mg·kg^(-1)剂量的毒死蜱,待雄性子鼠8周龄取左侧睾丸实施组织病理学检查,右侧睾丸用以检测丙二醛(maleic dialdehyde,MDA)的含量和谷胱甘肽S转移酶(glutathione S transferases,GST)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)的活力。结果表明,与对照组比较,随着染毒剂量的增加子鼠体重和睾丸、附睾脏器系数有下降的趋势(P>0.05);而MDA呈升高趋势(P>0.05)。各组T-SOD和1.35、2.70 mg·kg^(-1)剂量组GSH-Px活力的下降及2.70 mg·kg^(-1)剂量组GST活力的升高均有统计学意义(P<0.05)。睾丸组织病理学检查结果可见2.70mg·kg^(-1)剂量组睾丸组织有明显的损伤,管腔中精液量减少,生精细胞脱落增多。上述研究结果提示母鼠于围生期暴露于毒死蜱,可通过氧化损伤诱导子代雄性大鼠睾丸的毒性作用。

Chlorpyrifos is one of the largest used and saled organophosphorus pesticides throughout the world. To explore the testicular oxidative damage of 8 week offspring male rat induced by perinatal exposure to chlorpyrifos,the healthy pregnant Wistar female rats were exposed to chlorpyrifos of 0, 0.75, 1.35 and 2.70 mg·kg^（-1）by gavage during GD 6—PND21 d. Until the offspring male rat reached the age of 8 week, histopathological changes of left testis were observed. We also detected the level of MDA and the activity of GST, GSH-Px, and T-SOD in right testis. The results showed that although no statistical significance were observed in body weight, the organ coefficient of testis and epididymis compared with control group, both of them showed a downward trend and of MDA showedan upward trend when the dose increased（P〉0.05）. The decrease in the activity of T-SOD of all dose groups, the increase of the activity of GSH-Px of 1.35 mg·kg^（-1）and 2.70 mg·kg^（-1）dose groups and the increase in activity of GST of 2.70 mg·kg^（-1）dose group were statistically significant（P〈0.05）. The histopathological examination indicated that the testis of 2.70 mg·kg^（-1）dose group had significant damage. It is found that the semen volume was reduced and exfoliated spermatogenic cell was increased. Chlorpyrifos impaired the balance of antioxidant system,thus induced lipid peroxidation damage to the testis of 8 week rat by maternal exposure during GD 6 —PND21 d.It is proposed that chlorpyrifos had potential and long-term toxic effect to male reproductive system by perinatal exposure.