双酚AF对雄性斑马鱼卵黄蛋白原水平与芳香化酶基因表达的影响

Effects of Bisphenol AF on Level of Vitellogenin and Expressions of Cytochrome P450 Aromatase( cyp 19a and b) in Male Zebrafish

双酚AF(4,4'-六氟-2-二酚,BPAF)对生物有机体具有内分泌干扰作用。为研究低剂量BPAF对水生生物的效应,本研究选择成年雄性斑马鱼为研究对象,考察了0.005、0.05和0.5 mg·L^(-1)3种浓度BAPF暴露30 d对血浆中卵黄蛋白原(VTG)含量、2种卵黄蛋白原基因(vtg^(-1)和vtg-3)表达和2种芳香酶基因(cyp 19a与cyp 19b)表达的影响。结果表明:在0.005 mg·L^(-1)浓度暴露30 d后,血浆中VTG含量显著升高,随着暴露浓度的升高,促进作用不显著;BPAF暴露对不同组织中的4种基因存在不同的影响,0.005 mg·L^(-1)BPAF暴露可诱导脑部cyp19b、肝脏中cyp19a和性腺中vtg^(-1)、vtg-3和cyp19b基因表达;0.5 mg·L^(-1)BPAF暴露可导致肝脏中vtg^(-1)、vtg-3、性腺中cyp19a等基因显著上调。实验结果表明,BPAF具有雌性激素样效应,可诱导雄性斑马鱼体内部分组织卵黄蛋白原基因和芳香酶基因的表达。BPAF可引起斑马鱼血浆中的VTG含量的上升,从而干扰由VTG所参与的下丘脑-垂体-性腺轴与免疫系统的正常生理过程。

Bisphenol AF（4,4＇-hexafluoroisopropylidene-2-diphenol, BPAF） has endocrine disrupting effects on organisms. To study the effects of low dose of BPAF on organism in aquatic ecosystems, male adult zebrafish（Danio rerio） were exposed to 0.005, 0.05 and 0.5 mg·L^（-1）BPAF for 30 days. The level of vitellogenin（VTG） in plasma, the expression of two kinds of vtg m RNA（vtg^（-1） and vtg-3） and two kinds of aromatase genes（cyp19a and cyp19b） were analyzed after BPAF exposure. Results showed that a significantly high level of VTG could be in-duced by 0.005mg·L^（-1）BPAF for 30 day-exposure, but no significant upgrade of VTG was observed with the exposure to higher level of BPAF. Multiple effects were displayed by BPAF on four kinds of genes expression in different tissues. At 0.005mg·L^（-1）BPAF group, the BPAF markedly upregulated the expressions of cyp19 b in brain,cyp19 a in liver as well as vtg^（-1）, vtg-3 and cyp19 b in gonad. While treated with 0.5 mg·L^（-1）BPAF, the expressions of vtg^（-1） and vtg-3 m RNA in the brains and cyp19 a m RNA in the gonads were significantly increased. These results indicated that BPAF has estrogenic-like effects, which can induce the expression of both vtg genes and aromatase genes of the male zebrafish. BPAF can cause the increase of VTG content in the plasma of zebrafish,thereby interfering with the physiological processes of hypothalamus-pituitary-gonad axis and the immune system which VTG participates.