口服人参茎叶皂苷和黄芪多糖对鸡抗氧化功能的影响

Effects of ginseng stem-leaf saponins and Astragalus Polysacharin on oxidative stress induced by cyclophosphamide in chickens

研究饮水口服人参茎叶皂苷(GSLS)和黄芪多糖(APS)对环磷酰胺诱导鸡氧化应激的影响。将120只SPF鸡分为人参茎叶皂苷给药组(CY+GSLS)、阳性药物对照组(CY+APS)、CY对照组(CY+Saline)和空白对照组(Saline),比较口服GSLS和APS对氧化应激鸡抗氧化能力的影响。结果表明,氧化应激鸡口服GSLS和APS可显著提高鸡血清的总抗氧化能力(T-AOC),增加总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性,提高维生素C、维生素E和还原型谷胱甘肽(GSH)的水平,并降低血清中丙二醛(MDA)和蛋白质羰基碳(Carbonyl)的含量,提示GSLS和APS对氧化应激鸡抗氧化功能均具有显著提高作用,且GSLS拮抗鸡的氧化应激的作用较APS更为显著。

The present study was designed to evaluate the effects of ginseng stem-leaf saponins（GSLS） and Astragalus Polysacharin（APS） on oxidative stress induced by cyclophosphamide（CY） in chickens. 120 chickens were randomly divided into 4 groups. Groups A to C received intramuscular injection of CY to induce oxidative stress, while group D was injected with saline solution and served as control. Following administration of CY, group A was orally administrated GSLS at 5 mg/kg BW in drinking water for 7 days, group B was orally administrated APS at 200 mg/L in drinking water for 7 days. After that,the spleen, thymus, bursa and serum were collected to measure the indices of the organs and oxidative parameters. The results showed that GSLS and APS significantly inhibited CY-induced oxidative stress by increasing the organ indices, total antioxidant capacity（T-AOC）, and the levels of glutathione（GSH）, ascorbic acid（VC） and α-tocopherol（VE）, elevating the activity of total superoxide dismutase（T-SOD）, catalase（CAT） and glutathione peroxidase（GSH-Px）, as well as decreasing the protein carbonyl content and malondialdehyde（MDA）. And the effect of GSLS was better than that of APS.