云南省部分地区鸽粪中新型隐球菌格鲁比变种微卫星分型分析

Multilocus microsatellite typing of Cryptococcus neoformans var.grubii strains from pigeon droppings in part of Yunnan Province

目的对云南部分地区鸽粪分离株新型隐球菌进行多位点微卫星位点(multilocus microsatellite typing,MLMT)分型,了解其种群分布特征。方法对分离自云南省部分地区鸽粪中的152株新型隐球菌提取DNA,分别以CNG1、CNG2、CNG3为引物进行PCR扩增并测序,计算每一菌株CNG1中TA、CNG2中GA以及CNG3中CAT的重复数,结合不同基序重复数进行MLMT分型。结果采用MLMT法将大理、昭通、曲靖和临沧地区鸽粪分离株新型隐球菌格鲁比变种分为6个基因型,其中MLMT-17型占91.45%,为主要基因型;MLMT-26、29、34、39、40型分别占0.66%、1.97%、0.66%、0.66%和4.60%。结论 MLMT-17是云南省部分地区鸽粪新型隐球菌格鲁比变种中最常见的基因型。

Objective To use multilocus microsatellite typing（MLMT）to ascertain the genotype distribution of Cryptococcus neoformans var.grubii strains from pigeon droppings collected in parts of Yunnan Province. Methods DNA was extracted from 152 strains of C.neoformans var.grubii found in pigeon droppings.DNA fragments covering microsatellite loci（CNG1,CNG2,and CNG3）were amplified with PCR and sequenced.The number of repeated motifs in 3microsatellite regions was calculated.MLMT was used to type 152 strains of C.neoformans var.grubii depending the number of different repeated motifs. Results Six genotypes were identified in 152 strains from Yunnan Province.Of these strains,139 were MLMT-17,which accounted for 91.45% of the total strains.MLMT-26 accounted for 0.66% of the total strains,MLMT-29 accounted for 1.97%,MLMT-34 accounted for 0.66%,MLMT-39 accounted for 0.66%,and MLMT-40 accounted for 4.60%. Conclusion The MLMT-17 genotype of C.neoformans var.grubii was prevalent in pigeon droppings from parts of Yunnan Province.