微波消解-可见分光光度法测定紫甘薯中花青素

Determination of Anthocyanins in Purple Sweet Potato by Microwave Digestion and Visible Spectrophotometric

讨论紫甘薯中花青素的微波辅助提取及可见分光光度法定量检测方法。结果表明:在520 nm下测定样品中花青素的吸光度,相对标准偏差为5.26%(n=6),加标平均回收率为97.8%,最低检测浓度为0.835μg/m L。该方法简便准确,可用于紫甘薯中花青素含量的测定。

This article discuses the microwave-assisted extraction of anthocyanin from purple sweet potato and the way to quantitatively detect anthocyanin under visible spectrophotometer measure system.The result demonstrates that the determination of absrbance of anthocyanins in the sample at the 520 nm,the relative standard deviation is 5.26 %（n =6）,average recovery at the spiked level is 97.8 %,the minimum detectable concentration of anthocyanin is 0.835 μg/m L.The present invention provides a quick easy way to quantitatively detect anthocyanin in purple sweet potatoes.