摘要
以铁皮石斛为试材,以MS为基本培养基,研究了不同激素配比对铁皮石斛组培丛生芽增殖的影响。结果表明:铁皮石斛丛生芽增殖的培养条件为MS+6-BA 1.0mg/L+NAA 0.5mg/L+土豆汁100g/L+蔗糖30g/L,90d增殖系数达7.00,丛生芽芽高、茎径、含水量和叶绿素含量均高于其它处理。
Taking Dendrobium officinale as material,MS as basic media,the effect of different hormone ratio on bud proliferation of Dendrobium officinale was studied.The results showed that the most suitable medium of Dendrobium officinale buds proliferation was MS＋6-BA 1.0mg/L＋NAA 0.5mg/L＋potatoes 100g/L＋sucrose 30g/L,the proliferation coefficient was 7.00 after 90days;bud height,stems diameter,moisture content and chlorophyll content were higher than other treatments.
出处
《北方园艺》
CAS
北大核心
2016年第6期106-108,共3页
Northern Horticulture
基金
"贵州珍稀药材石斛
白及品种选育及其产业化"资助项目(黔农科院自主创新科研专项字(2014)017号)
贵州省农业委员会资助项目(GZCYTX2014-0202)
贵州省中药现代化科技专项资助项目(黔科合中药字[2013]5033号)
关键词
铁皮石斛
丛生芽
增殖培养
Dendrobium officinale
buds
proliferation culture
