bta-mir-423-5p在胎衣不下奶牛母体胎盘中的差异表达分析及验证

Analysis and validation of differential expression of bta-mir-423-5p in placenta of cow with retained fetal membranes

目的分析及验证bta-mir-423-5p在胎衣不下奶牛母体胎盘中的差异表达。方法选取各种因素无明显差异,且排除其他疾病影响的胎衣正常排出奶牛(对照组)和胎衣不下奶牛(试验组)各3头,对照组在奶牛产后胎衣排出后,立即采集母体胎盘组织约150 mg,试验组在奶牛产后12 h,收集母体胎盘组织约150 mg。采用Solexa高通量测序技术对两组母体胎盘组织间bta-mir-423-5p的差异表达进行分析;利用Real-time Q-PCR法进行验证。结果试验组奶牛母体胎盘中bta-mir-423-5p的表达量明显低于对照组(P<0.01)。结论 bta-mir-423-5p在胎衣不下奶牛母体胎盘中异常表达,表明其可能参与胎衣不下的发生,为奶牛胎衣不下发病机理的研究提供了实验依据。

Objective To analyze and validate the differential expression of bta-mir-423-5p in placenta of cow with retained fetal membranes（RFM）. Methods The placenta tissues of three cows with RFM（test group） and three normal cow placenta tissues（control group）were selected. About 150 mg of samples were collected in control group immediately after placental expulsion, while about 150 mg in test group 12 h after birth of calf. The differential expression of bta-mir-423-5p in placenta tissues of the two groups were analyzed by high throughput sequencing（Hi Seq）, and validated by realtime Q-PCR. Results The expression level of bta-mir-423-5p in test group was significantly lower than that in control group（P 〈0. 01）. Conclusion The abnormal expression of bta-mir-423-5p in maternal placenta indicated that bta-mir-423-5p might participate in the occurrence of RFM, which provided an experimental basis for study on the pathogenic mechanism of RFM.