抗促黄体激素释放激素受体单克隆抗体对人子宫内膜癌Ishikawa细胞增殖及凋亡的影响

Effect of monoclonal antibody against luteinizing hormone-releasing hormone receptor on proliferation and apoptosis of human endometrial carcinoma Ishikawa cells

目的探讨抗促黄体激素释放激素受体(luteinizing hormone-releasing hormone receptor,LHRHR)单克隆抗体4F3B10对人子宫内膜癌Ishikawa细胞增殖及凋亡的影响。方法利用半定量RT-PCR分析Ishikawa细胞中LHRHR基因m RNA的转录水平,以β2-M基因为内参标准,通过光密度定量扫描比较确定LHRHR基因量。采用CCK-8法检测4F3B10对Ishikawa细胞增殖的影响;利用Annexin V-FITC/PI双染,在流式细胞仪上检测4F3B10对Ishikawa细胞凋亡的影响。结果 Ishikawa细胞中LHRHR的基因量约为内参的67.23%。4F3B10对Ishikawa细胞的增殖具有抑制作用,且呈剂量依赖性;经半数抑制浓度的4F3B10作用Ishikawa细胞48 h后,细胞凋亡率较未经4F3B10作用的细胞显著升高(P<0.05)。结论 4F3B10可有效抑制Ishikawa细胞增殖,并可诱导其凋亡。

Objective To investigate the effect of monoclonal antibody（Mc Ab） 4F3B10 against luteinizing hormonereleasing hormone receptor（LHRHR） on proliferation and apoptosis of human endometrial carcinoma Ishikawa cells.Methods The transcription level of LHRHR m RNA in Ishikawa cells was determined by semi-quantitative RT-PCR. The quantity of LHRHR gene was determined by comparing its optical density with that of β2-M gene as an internal reference.The effect of 4F3B10 on proliferation of Ishikawa cells was evaluated by CCK-8 method, while that on apoptosis by flow cytometry with Annexin V-FITC / PI staining. Results The quantity of LHRHR gene in Ishikawa cells was about 67. 23%of that of β2-M gene. 4F3B10 showed dose-dependent inhibitory effect on the proliferation of Ishikawa cells. The apoptosis rate of ishikawa cells 48 h after treatment with 4F3B10 at median inhibitory concentration increased significantly compared with that untreated（P 〈0. 05）. Conclusion 4F3B10 inhibited the proliferation and induced the apoptosis of Ishikawa cells effectively.