摘要
目的对比分析两种检测人抗凝血酶-Ⅲ（antithrombin-Ⅲ,AT-Ⅲ）活性的方法。方法分别用手工法和仪器法检测AT-Ⅲ活性,手工法根据《欧洲药典》8.0中AT-Ⅲ含量检测2.7.17项的要求进行检测,仪器法是利用法国STAGO全自动血凝仪及其配套试剂进行检测。同时对两种方法检测结果的直线回归相关系数、重复性、准确性进行验证。结果两种方法的直线回归相关系数均〉0.99,RSD均〈10%,回收率均在80%~120%之间。结论两种方法的一致性良好,且仪器法更省时省力,效率更高。
Objective To compare two methods for determination of activity of human antithrombin-Ⅲ（AT-Ⅲ）. Methods The activity of human AT-Ⅲ was determined by manual method according to the requirements in section 2. 7. 17 of European Pharmacopoeia 8. 0, and by instrument method using French STAGO automatic blood coagulation analyzer and the matching reagent respectively. The determination results by two methods were verified for correlation coefficient of linear regression, reproducibility and accuracy. Results Both the linear regression correlation coefficients of the two methods were more than 0. 99, while the RSDs were less than 10%, and the recovery rates were 80% ~ 120%. Conclusion The two methods showed good consistency. However, the instrument method was time- and labor-saving, and more effective.
出处
《中国生物制品学杂志》
CAS
CSCD
2016年第3期304-307,共4页
Chinese Journal of Biologicals
关键词
人抗凝血酶-Ⅲ
活性
仪器法
手工法
Human antithrombin-Ⅲ
Activity
Instrument method
Manual method
