六味地黄丸对自发性2型糖尿病模型小鼠胰岛素表达水平的影响

Effects of Liuweidihuang pill on insulin levels in sera and pancreatic islets from spontaneous mouse models of human type 2 diabetes administrated with different doses

目的：探讨不同剂量六味地黄丸对自发性2型糖尿病模型小鼠血清和胰岛内胰岛素表达水平的影响。方法：将6～8周龄自发性2型糖尿病模型小鼠（KK-Ay小鼠）随机分3组（无药对照组、低剂量组、高剂量组）,C57BL/6J小鼠作为遗传对照组,各组小鼠分别给予蒸馏水或六味地黄丸灌胃15周。每周测定空腹血糖、体质量、摄食量;给药15周后,ELISA法测定小鼠外周血血清胰岛素含量,免疫荧光法和免疫组织化学法检测胰岛中胰岛素表达水平,比较各组检测结果。结果：六味地黄丸给药后,KK-Ay小鼠空腹血糖降低,体质量和摄食量得到控制;给药组小鼠血清胰岛素含量和胰岛内胰岛素表达水平明显降低,且低剂量组的降低程度比高剂量组更明显。结论：六味地黄丸对自发性2型糖尿病模型小鼠高血糖、肥胖、胰岛素抵抗具有一定的治疗作用,且低剂量组降低血清和胰岛内胰岛素水平的效果优于高剂量组。

Objective： To investigate the effects of Liuweidihuang pill on the insulin levels in sera and pancreatic islets from spontaneous mouse models of human type 2 diabetes administrated with different doses. Methods： The 6-8 week-old KK-Ay mice were randomly divided into three groups including no drug control group,low-dose group and high-dose group,in addition C57 BL /6J mice were used as a genetic control group. All the animals were given with different dose Liuweidihuang pill solutions or sterile distilled water by intragastrical administration for fifteen weeks. The fasting blood glucose,body mass and food consumption were measured weekly. The serum insulin levels were surveyed by ELISA. And the insulin levels in the pancreas islets were detected by immunofluorescence and immunohistochemistry. Results： Decreased fasting blood glucose,controlled body mass and food consumption,and lower levels of insulin in the sera and pancreas islets were confirmed from the KK-Ay mice administered with Liuweidihuang pill. Furthermore,the low dose program exhibits a stronger effect. Conclusion： Liuweidihuang pill has exhibited relatively therapeutic effects in the spontaneous type 2diabetes mice including controls of hyperglycemia and body mass and relieving insulin resistance. In addition,the low-dose regimen showed even better treatment in controlling insulin levels in the sera and pancreas islets.