过继转移实验性自身免疫性葡萄膜炎模型光相干断层扫描与病变程度评分以及组织病理学评价的比较

Comparison of optical coherence tomography with clinical and histopathological findings in adoptively transferred uveitis in mice

目的观察探讨过继转移自身免疫性葡萄膜炎模型（EAu）小鼠光相干断层扫描（OCT）检查结果与病变程度评分和病理组织检查结果的一致性及其意义。方法C57BL／6小鼠腹腔注射抗原特异性T细胞建立过继转移EAU动物模型。过继免疫后9d开始，每3天观察1次直至过继免疫后60d，间接检眼镜联合前置镜观察小鼠眼底炎症情况并进行评分。将病变程度分为发病初期、发病早期、高峰前期、高峰期、恢复期、末期，相应评分分别为0．5、1.0、1．5～2．0、2．5～3．0、1～2分以及小于1分。过继免疫后9、11、16、21、26、30、35、40、50、60d采用海德堡SpectralisOCT仪测量距视盘边缘约1个视盘直径范围的视网膜厚度（RT）。依据OCT检查结果将病变程度分为0．O～4．0分。过继免疫后9、21、60d处死小鼠，摘取眼球行组织病理学检查。对比分析各检查时间点OCT图像特征与眼底病变程度评分和组织病理学检查结果的一致性。结果过继免疫后9、16、21、26d分别为EAU发病初期、发病早期、高峰前期和高峰期，OCT评分分别为0．5、1．0、2．0、4．0分；30d为恢复期，可见玻璃体炎症细胞减少，OCT评分平均为3．0分；60d为末期，玻璃体炎症细胞浸润消退，视网膜萎缩，RT降低，OCT评分0．5分。光学显微镜观察，发病初期、发病早期玻璃体腔有少量炎症细胞浸润，视网膜结构相对正常；高峰前期视网膜水肿、玻璃体腔可见炎症细胞及视网膜结构紊乱；恢复期玻璃体腔可见极少量炎症细胞，视网膜萎缩变薄。相关性分析结果显示，OCT评分和病变程度评分高度相关（r=0．9579，P〈0．0001）。结论过继转移EAu模型小鼠OCT检查结果与其病变程度评分和病理组织检查结果具有较好的一致性；OCT检查结果有助于动态量化评估EAu模型小鼠病变程度。

Objective To explore the consistency and significance of optical coherence tomography （OCT） and clinical and histopathological findings in adoptively transferred uveitis in mice. Methods The adoptively transferred experimental autoimmune uveitis （EAU） model was established by intraperitoneal injection of antigen-specific T cells in C57BL/6 mice. Since 9 days after transferred, inflammation of eyes was observed by indirect ophthalmoscope with ＋ 90D lens and record clinical scores every 3 days. The disease was divided into 6 phases including onset phase, early phase, pre-peak phase, peak phase, resolution phase and late phase of EAU, which respectively corresponding to clinical score 0.5, 1.0, 1.5 - 2.0, 2.5 - 3.0, 1.0 - 2.0 and less than 1.0. Since 9 days after transferred, the retina and retinal thickness （RT） was measured by spectralis OCT about 1 disc from the disc edge in 10 time points including 9, 11, 16, 21, 25, 30, 35, 40, 50 and 60 days after transferred. The OCT score was recorded as from 0.0 to 4.0. After transferred 9, 21 and 60 days, the mice were killed and eye balls were examined in histology. OCT score, clinical score and histology in the mouse were compared and analyzed. Results The disease was divided into onset phase, early phase, pre-peak phase and peak phase of EAU, which respectively corresponding to 9, 16, 21 and 26 days after transferred. In four phases, OCT score were 0. 5, 1.0, 2.0 and 4.0 respectively. After transferred 30 days, which was in resolution phase of EAU, the inflammation cells in vitreous were decreased and OCT score was 3.0. After transferred 60 days, which was in late phase of EAU,inflammation cells in vitreous were disappeared and relina was atrophic topically. The histology showed the vitreous has slight inflammation cells and retinal structure was normal at onset of EAU. The vitreous has massive inflammation cells and retina slructure was disorder at pre peak of EAU. And in resolution phase of EAU, the inflammation cells in vitreous were slightly and retire1 was atrophic and thinned. The data in this study demonstrated that （XCT score was well correlated with clinical score in EAU （r=0. 957 9, P〈0. 000 1 ）. Conclusion OCT and clinical and histopathological findings in adoptively transferred uveitis in mice were consistency and OCT is contribute to evaluate the disease dynaically and quantifiably.