摘要
Six main mitochondrial DNA （mtDNA） lineages have been described in minnow （Zacco platypus） samples obtained from northern, western and southern China Perdices et al. （2004） predicted that further sampling of other tributaries might discover more lineages of this species. In this study, we collected 26 Zacco platypus individuals in the Huangshan area of eastern China and determined the cytochrome b （cytb） sequence variations. Combined with reported data in GenBank, we identified ten matrilines （Zacco A-J） in a total of 169 samples, with relatively high molecular divergence found among them. The Huangshan population had the greatest genetic variation among all sampled regions and hosted six of the ten matrilines. Our results highlight the significance of the Huangshan area for the conservation of Zacco platypus.
Six main mitochondrial DNA （mtDNA） lineages have been described in minnow （Zacco platypus） samples obtained from northern, western and southern China Perdices et al. （2004） predicted that further sampling of other tributaries might discover more lineages of this species. In this study, we collected 26 Zacco platypus individuals in the Huangshan area of eastern China and determined the cytochrome b （cytb） sequence variations. Combined with reported data in GenBank, we identified ten matrilines （Zacco A-J） in a total of 169 samples, with relatively high molecular divergence found among them. The Huangshan population had the greatest genetic variation among all sampled regions and hosted six of the ten matrilines. Our results highlight the significance of the Huangshan area for the conservation of Zacco platypus.
基金
Foundation items： This research was funded by the National Natural Science Foundation of China （NSFC 30870290, 31071891 and 31471968）ACKNOWLEDGEMENTS We extend our thanks to Dr. Robert W. MURPHY for his valuable comments on this manuscript. We thank Jin-Min CHEN （Yunnan University）, Bao-Lin ZHANG （Kunming Institute of Zoology, CAS） and Li-Fang PENG （Nanjing Forestry University） for their help in data processing. We further thank Dian- Cheng YANG （Nanjing Forestry University） and Jun-Sheng CUI （Anhui Agricultural University） for sampling.
