摘要
旨在探究BVDV感染对牛外周血来源的树突状细胞成熟及Th1和Th2型细胞因子分泌水平的影响,进一步揭示BVDV感染对机体免疫机能的影响机制。从牛外周血分离单核细胞,诱导培养为树突状细胞后接种BVDV共培养,采用显微镜观察细胞的形态,流式细胞术分析细胞表面标志分子,半定量RT-PCR法检测Th1型细胞因子IL-12p40和Th2型细胞因子IL-10的mRNA转录水平。结果显示,获得形态及表型典型的树突状细胞,诱导纯度为93.81%;BVDV接种组树突状细胞表面的MHCⅡ类分子、CD40分子、CD80分子均明显低于未接种组,差异极显著(P<0.01);BVDV接种组的IL-12p40 mRNA转录水平低于未接种组,差异显著(P<0.05);而IL-10mRNA转录水平高于未接种组,差异显著(P<0.05)。结果说明BVDV感染影响树突状细胞的成熟及功能,并能通过高效表达Th2型细胞因子IL-10、降低Th1型细胞因子IL-12p40而影响Th1/Th2的平衡。
The objective of this study was to investigate the influence of BVDV on the cell maturation and cytokine level of Th1 and Th2type in dendritic cells（DC）derived from bovine peripheral blood,and to further reveal the immune mechanism of BVDV infection.The monocytes from bovine peripheral blood were separated and induced into DCs,and then were co-cultured with BVDV.The cell morphology and the expression of MHCⅡ,CD80 and CD40on DCs were observed by microscopy and flow cytometry respectively,the semi-quantitative RT-PCR was used to analyze IL-12p40 of Th1type cytokines and IL-10 of Th2at mRNA levels.The results showed that DCs had typical morphology and immunophenotype with 93.81% purity.Post BVDV infection,the MHCII molecules,CD40 molecules and CD80 molecules were greatly lower than that of the control groups,the differences were statistically significant（P〈0.01）.IL-12p40 mRNA level was down-regulated,while IL-10 mRNA level was up-regulated,the differences were significant（P〈0.05）compared with the control groups.These results indicated that BVDV infection to DC plays an important role on maturation and regulating the balance of Th1/Th2.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2016年第3期536-542,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
辽宁省博士启动项目(20141057)
大学生科技创新项目(201339)
