不同浓度的普伐他汀对小鼠巨噬细胞极性的影响研究

Effect of different concentrations of pravastatin treatment on macrophage polarity in mice

目的探讨不同浓度的普伐他汀对小鼠巨噬细胞极性的影响。方法对小鼠骨髓来源的巨噬细胞进行体外培养,以0μmol/L普伐他汀钠组作为对照组,分别给予10、25、50μmol/L的普伐他汀钠进行药物干预24h,用酶联免疫吸附试验(ELISA)检测白细胞介素(IL)-10、IL-12的分泌,流式细胞仪检测细胞膜CD16/32、CD206的表达,荧光定量聚合酶链反应(PCR)检测Toll样受体4(TLR4)、髓样分化因子88(MyD88)、干扰素调控因子5(IRF5)mRNA的表达。结果普伐他汀钠干预后的巨噬细胞,随着普伐他汀钠的浓度升高,IL-12、CD16/32的表达下降,而IL-10、CD206的表达升高,并伴有TLR4、MyD88、IRF5mRNA的表达下调,且呈剂量依赖性。结论普伐他汀钠促进巨噬细胞向抗炎性M2型极化,该效应可能与普伐他汀钠的抗炎作用有关。

Objective To study the effect of different concentrations of pravastatin treatment on macrophage polarity in mice.Methods The mice bone marrow sources of macrophages were cultured in vitro,with the 0μmol/L sodium pravastatin group as control,by giving 10,25,50μmol/L sodium pravastatin to conduct the drug intervention for 24 h.The enzyme linked immunosorbent assay（ELISA）was used to detect the secretion of interleukin 10（IL-10）and IL-12;the flow cytometry instrument was used to detect cell membrane CD16/32,CD206expression;real time quantitative polymerase chain reaction（RT-qPCR）was adopted to detect toll-like receptor 4（TLR4）,myeloid differentiation factor 88（MyD88）,interferon regulatory factor 5（IRF5）mRNA expression.Results After the intervention of pravastatin sodium on macrophages,as the pravastatin sodium concentration increase,the expression of IL-12 and CD16/32 was decline,while the expression of IL-10 and CD206 was risen,which was accompanied by TLR4,MyD88,IRF5 mRNA expression down regulatyion,and a dose dependent manner.Conclusion Sodium pravastatin promote the polarization of macrophages toward an anti-inflammatory macrophage phenotype（M2）,this effect may be related with the antiinflammatory effect of sodium pravastatin.