梁山慈竹UGP基因克隆与生物信息学分析

Cloning and Bioinformatics Analysis of Df UGP Gene in Dendrocalamus farinosus

以梁山慈竹笋为材料,采用RT-PCR方法克隆梁山慈竹UGP基因,对梁山慈竹UGP基因的编码区、氨基酸序列及蛋白质的结构和功能进行生物信息学分析。获得1个梁山慈竹UGP基因,命名为Df UGP（Genebank：KJ525752）。测序结果显示该序列全长1561 bp,包含一个完整的ORF框,长度为1422 bp,编码473个氨基酸。梁山慈竹UGP基因与巨龙竹、绿竹、水稻、大麦、玉米、甘蔗的具有高度的同源相似性,编码的蛋白结构较为相似,均含有N端区域、中心区域和C端区域,且蛋白三级结构中都具有NBloop环和l-loop环。Df UGP具有N端的UDPGP功能域,其位于第90-379氨基酸,证明Df UGP属于尿苷二磷酸葡萄糖焦磷酸化酶基因家族。

Taken Dendrocalamusfarinosus as tested materials, the young shoots of Dendrocalamusfarinosus was used to clone the UCP gene by using RT-PCR, and the encoding region and amino acid sequence of UGP gone and the structure and function of protein encoded with UGP gene were analyzed by NCBI, ExPASy. The result showed that the UGP gone was cloned in Dendrocalamusfatinosus, which was named DfUGP （GenBank： KJ525752）. Its full-length of nucleotide sequence was 1561 bp, containing a complete open reading frame with 1422 bp, which encoded 473 amino acids. Phylogonetic analysis showed that the UGP gene from Derutrocalamusfarinosus was highly homologous to the Dendrocalaraus sinicus, Bambusa oldhamii, Oryza sativa, Hordeum vulgare, Zea mays and Saccharum officinarum, and the NB-lcop rings and l-loop rings were found in the tertiary structure of UGP. DfUGP had the N-terminal domain, located at 90-379 amino acids, which indicated that DfUGP gene belonged to uridine diphosphate glucose phosphorylase gone families.