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电子嗅在线反馈控制毕赤酵母糖化酶发酵过程中甲醇浓度新方法的应用 被引量:1

Application of the Electronic Nose on the Online Feedback Control of Methanol Concentration during Glucoamylase Fermentation Optimization by Pichia pastoris
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摘要 探索了电子嗅传感仪直接通过发酵尾气进行发酵液中甲醇浓度在线检测的方法,建立了毕赤酵母表达糖化酶过程中甲醇浓度的自动化反馈补料控制模型,可准确实现发酵过程中甲醇浓度的精确控制;研究表明,当利用电子嗅将培养液中甲醇浓度稳定控制在(890±35)ppm水平下,发酵诱导培养到128h时目的蛋白糖化酶酶活达到了8 153U/ml,与甲醇浓度控制在(350±26)ppm时的发酵水平相比提升了48.8%。该方法具有无需前处理、与发酵液非接触、快速和准确性的优点,为提升工程酵母在工业发酵培养过程工艺的优化控制具有重要的指导作用。 A novel method for direct determining the broth methanol concentration online from the fermentation exhausted gas with electronic nose sensor, established an auto-feedback model for methanol concentration control during glucoamylase fermentation by genetic Pichia pastoris were investigated,the model could be used for precise control in the fermentation process. Methanol concentration controlling strategies through electronic nose showed that when the broth methanol concentration in stability controlled at( 890 ± 35)ppm,the highest glucoamylase activity reached 8 153 U / ml at 128 h of fermentation,which was 48. 8% higher than that with the methanol concentration controlled at( 350 ± 26) ppm. Online methanol detection and feedback control models needn't pre-treatment and contact with the broth,has the rapid and accuracy advantages. It will be approved as an effective and important role on the online methanol concentration analysis and feedback control on industrial enzyme or aim protein fermentation with genetic Pichia pastoris.
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2016年第3期68-76,共9页 China Biotechnology
基金 国家"863"计划资助项目(2015AA021005)
关键词 电子嗅 甲醇 在线监测 毕赤酵母 糖化酶 Electronic nose Methanol Online determination Pichia pastoris Glucoamylase
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  • 1船津 胜鹤 等.溶菌酶(第1版)[M].山东科学技术出版社,1982.256-293.
  • 2张珍田 陈良惠 等.-[J].利用超过滤法纯化鸡蛋白溶菌酶之研究,1986,24(2):86-93.
  • 3B.施特尔 马赫.酶的测定方法(第1版)[M].中国轻工业出版社,1992.236-241.
  • 4吴晃荣.微生物菌种保存法[M].华香园出版社,中华民国七十四年.218.
  • 5ZHOU X S(周祥山) FAN W M(范卫民) ZHANG Y X(张元兴).Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris[J].Chinese Journal of Biotechnology (生物工程学报),2002,18(3):348-351.
  • 6Ql Y Z(戚以政) WANG S X(汪叔雄).Biochemical reaction dynamics and reactor(生化反应动力学与反应器)[M].Peking:Chemical Engineering Press,1996..
  • 7Hasslacher M, Schall M, Hayn M et al. High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts. Protein Expr Purif, 1997, 11:61 -71.
  • 8Werten M M, van den Bosch T J, Wind R D et al. High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast, 1999,15: 1087- 1096.
  • 9Zhang W H, Bevins M A, Plantz B A et al. Modeling Pichia pastoris growth on methanol and optimization the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng, 2000,70:1-8.
  • 10d' Anjou M C, Daugulis A. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol Bioeng, 2001, 72:1 - 11.

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