摘要
探索了电子嗅传感仪直接通过发酵尾气进行发酵液中甲醇浓度在线检测的方法,建立了毕赤酵母表达糖化酶过程中甲醇浓度的自动化反馈补料控制模型,可准确实现发酵过程中甲醇浓度的精确控制;研究表明,当利用电子嗅将培养液中甲醇浓度稳定控制在(890±35)ppm水平下,发酵诱导培养到128h时目的蛋白糖化酶酶活达到了8 153U/ml,与甲醇浓度控制在(350±26)ppm时的发酵水平相比提升了48.8%。该方法具有无需前处理、与发酵液非接触、快速和准确性的优点,为提升工程酵母在工业发酵培养过程工艺的优化控制具有重要的指导作用。
A novel method for direct determining the broth methanol concentration online from the fermentation exhausted gas with electronic nose sensor, established an auto-feedback model for methanol concentration control during glucoamylase fermentation by genetic Pichia pastoris were investigated,the model could be used for precise control in the fermentation process. Methanol concentration controlling strategies through electronic nose showed that when the broth methanol concentration in stability controlled at( 890 ± 35)ppm,the highest glucoamylase activity reached 8 153 U / ml at 128 h of fermentation,which was 48. 8% higher than that with the methanol concentration controlled at( 350 ± 26) ppm. Online methanol detection and feedback control models needn't pre-treatment and contact with the broth,has the rapid and accuracy advantages. It will be approved as an effective and important role on the online methanol concentration analysis and feedback control on industrial enzyme or aim protein fermentation with genetic Pichia pastoris.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2016年第3期68-76,共9页
China Biotechnology
基金
国家"863"计划资助项目(2015AA021005)
关键词
电子嗅
甲醇
在线监测
毕赤酵母
糖化酶
Electronic nose Methanol
Online determination
Pichia pastoris
Glucoamylase