离子选择电极法与偶氮胂Ⅲ法检测血钙的比较研究．

Comparison of ion selectivity electrode assay and arsenazo Ⅲ aSsay in determination serum calcium concen-tration

目的比较离子选择电极法与偶氮胂Ⅲ法检测血钙浓度的差异，探讨两种检测方法的相关性。方法采集142例患者的血清标本，分别采用离子选择电极法和偶氮胂Ⅲ法进行检测，比较两种方法检测血钙浓度的差异，采用person相关分析及Bland—Altman分析探讨两种检测方法的相关性。结果离子选择电极法测定血钙浓度为（2．23±0．10）mmol／L，偶氮胂Ⅲ法测定血钙浓度为（2．24±0．12）mmol／L，两种方法检测结果差异无统计学意义（t=-1．09，P〉0．05）；Person相关分析显示，两种方法检测结果存在显著正相关关系（r=0．393，P〈0．01）；Bland—Altman分析表明，两种方法检测结果具有较好的一致性。结论离子选择电极法与偶氮胂Ⅲ法检测血钙浓度比较，两种方法具有较好的一致性。

Objective To evaluate the correlation of the ion selectivity electrode assay and arsenazo Ⅲ assay for serum calcium determination and compare the differences between two methods. Methods 142 serum samples were collected from patients and detected by ion selectivity electrode assay and arsenazo Ⅲ assay, the differences of two methods were compared, person correlation and Bland - Ahman was used to discuss the correlation. Results The serum calcium concentration detected by ion selectivity electrode assay was （2.23± 0.10）mmol/L and detected by arsenazo Ⅲ assay was （2.24 ± 0.12）mmol/L, the difference between the two methods was not statistically significant （t = -1.09 ,P 〉0.05）. The person correlation analysis demonstrated that the two methods had a positive correlation （ r = 0. 393, P 〈 0.01 ）. The Bland - Altman consistency analysis showed that ion selectivity electrode assay was agreed with arsenazo HI assay. Conclusion The two methods for serum calcium concentration determination, the ion selectivity electrode assay was coincidence with the arsenazo Ⅲ assay.