摘要
目的探讨腺病毒介导白细胞介素-24基因对瘢痕疙瘩成纤维细胞中纤溶酶原激活物抑制剂-1和Ⅰ、Ⅲ型胶原蛋白mRNA及蛋白水平表达的影响。方法构建白细胞介素-24腺病毒载体，感染瘢痕疙瘩成纤维细胞，并设置阴性对照组和空白对照组。应用实时定量PCR检测纤溶酶原激活物抑制剂-1及Ⅰ、Ⅲ胶原蛋白mRNA表达的变化，WesternBlot检测纤溶酶原激活物抑制剂-1及Ⅰ、Ⅲ型胶原蛋白水平表达的变化。结果白细胞介素-24腺病毒载体感染瘢痕疙瘩成纤维细胞后，与阴性对照组相比，感染组纤溶酶原激活物抑制剂-1和Ⅰ、Ⅲ型胶原蛋白mRNA及蛋白表达量降低（P〈0．05）。空白对照组与阴性对照组差异无统计学意义（P〉0．05）。结论白细胞介素-24腺病毒载体可能通过调节纤溶酶原激活物抑制剂．1及Ⅰ、Ⅲ型胶原蛋白mRNA及蛋白的表达，减少瘢痕疙瘩成纤维细胞胶原等细胞外基质的沉积。
Objective To investigate the effect of IL-24 gene on expression on mRNA and protein level of Plasminogen activator inhibitor-1 （ PAI-1 ） , type I collagen and type 111 collagen of human keloid fibrobl asts （KFs）. Methods Interleukin 24 （IL-24） gene was cloned into adenovirus vector, then the adenovirus particles expressing IL-24 were infected into keloid fibroblasts （KF cells ） , KF-IL-24; KF-NC and KF-CON were used as the control groups. Real time PCR and Western blot were performed to examine the expression of IL-24 in adenovirus infected cells. Results Compared with KF-CON group and KF-NC group, the expression levels of IL- 24 mRNA and protein were both significantly upregulated in KF-IL-24 group after KF cells were infected （ P 〉 0.05 ）. The difference between the KF-CON group and KF-NC group was not statistically significant （ P 〉 0.05 ）. Conclusion Adenovirus-mediated IL-24 gene may inhibit deposition of extracellular matrix of KF through con- trolling the expressions of PAI-1, type Ⅰ collagen and type Ⅲ collagen mRNA and protein.
出处
《中国美容整形外科杂志》
CAS
2016年第3期177-180,共4页
Chinese Journal of Aesthetic and Plastic Surgery
基金
广东省省级科技计划项目（20138021800063）
