糖尿病肾病患者血清VEGF与微量白蛋白尿的相关性研究

The correlation study between serum VEGF and microalbuminuria in the diabetic nephropathy

目的探讨糖尿病肾病(DN)患者血清中血管内皮生长因子(VEGF)水平的变化及其与尿微量白蛋白的关系,为DN蛋白尿提供新的治疗方向。方法 54例糖尿病(DM)患者,依据24 h尿白蛋白定量将其分为3组,即正常蛋白尿组、DM微量蛋白尿组和DN组;20例健康体检者为正常对照组。采用双抗体夹心酶联免疫吸附法(ELISE)检测各组血清中VEGF的水平,分析各组血清VEGF含量表达的变化及其与尿微量白蛋白等临床指标的相关性。结果与对照组相比,DM微量蛋白尿组及DN组血清VEGF水平明显升高(P<0.05);与DM正常蛋白尿组比较,DN组血清中VEGF的表达水平明显升高(P<0.05);血清VEGF的水平与空腹血糖(r=0.629,P<0.05)、尿微量白蛋白(r=0.293,P<0.05)呈显著正相关性,与糖化血红蛋白、糖化血清蛋白、血尿酸、血肌酐、尿素氮、血压无明显相关性。结论 DN患者血清中VEGF的表达异常升高,并且与尿微量白蛋白呈正相关关系;抑制VEGF的过度表达,可能是一种防治蛋白尿形成的重要手段。

Objective To explore the relationship between the changes in the expression levels of serum VEGF and microalbuminuria in DN for providing a new treatment direction. Methods According to the 24 h urine albumin quantitative,54 cases of diabetes with or without albuminuria were divided into three groups： non albuminuria group,microalbuminuria group,and DN group. Twenty healthy people were selected as control group. The levels of serum VEGF in different groups were detected by ELISA. The levels of serum VEGF changes were analyzed in each group and the correlation with urinary albumin and other clinical indicators were analyzed.Results Comparedwith control group,the serum VEGF level was significantly increased in the micro and DN groups（ P〈0. 05）. Compared with the nonalbuminuria group,the serum VEGF level was significantly increased in DN group（ P〈0. 05）. The serum VEGF level was significantly correlated with fasting blood glucose（ r = 0. 629,P〈0. 05） and urine microalbumin（ r = 0. 293,P〈0. 05）. There was no correlated with serum creatinine,urea nitrogen,uric acid,glycated protein,systolic and diastolic blood pressure. Conclusion The serum VEGF level is significantly increased in DN patients,and the VEGF level has positive correlation with microalbuminuria. It may provide a new sight for the prevention and treatment of DN.