2型糖尿病肾病患者TGF-β1水平与蛋白尿的关系

The relationship of serum TGF- β1 expression and albuminuria in type 2 diabetic nephropathy

目的探讨转化生长因子-β1（TGF-β1）在2型糖尿病肾病（DN）患者血清中的含量及与蛋白尿之间的关系。方法将62例2型糖尿病患者,根据尿白蛋白定量分为3组,即DM无蛋白尿组（尿白蛋白定量〈20μg/min）、DM微量蛋白尿组（尿白蛋白定量20~200μg/min）、DM大量蛋白尿组（尿白蛋白定量〉200μg/min）。20例健康体检者为正常对照组,男13例,女7例,平均年龄（66.10±10.01）岁。收集受试者空腹血样,应用ELISA法检测血清中TGF-β1的水平,收集、分析相关临床实验室资料。结果与正常对照组、糖尿病无蛋白尿组相比,血清TGF-β1在DM微量蛋白尿组中升高（P〈0.05）,在DM大量蛋白尿组中明显升高（P〈0.05）;血清TGF-β1表达水平与空腹血糖、糖化血红蛋白、糖化血清蛋白、尿微量白蛋白呈正相关性。结论 DN患者血清TGF-β1水平可随高糖环境诱导增加,并与尿微量蛋白量呈正相关性。

Objective To explore the relationship of TGF- β1（ transforming growth factor,TGF- beta 1） and albuminuria in patients with type 2 diabetic nephropathy. Methods According to the quantitative of urinary albumin,sixty- two patients with type 2diabetic mellitus（ T2DM） were divided into three groups： DM without albuminuria（ albuminuria level 20 μg / min）; DM with trace albuminuria（ albuminuria level between 20 ~ 200 μg / min） and DM with massive albuminuria（ albuminuria level 200 μg / min）. Twenty sex and age- matched healthy volunteers were recruited as control group. There were 13 male and 7 female with an average of 66. 10 ±10. 01. The expression of serum TGF- β1 was measured by enzyme linked immunosorbent essay（ ELISA）. All clinical and laboratory data were collected and analyzed. Results Compared with the DM without albuminuria group and normal group,the expression of serum TGF- β1 was significantly increased in DM with albuminuria group（ P〈0. 05）. The level of serum TGF- β1 had positive correlation with fasting blood protein,glycosylated hemoglobin,glycated serum protein and the quantitative of urinary albumin. Conclusion Hyperglycemia can increase the expression of serum TGF- β1 and TGF- β1 had positive correlation with albuminuria in type 2 diabetic nephropathy.