尿素包合法提纯紫苏籽油中α-亚麻酸工艺的优化

Purification Technique Optimization of α-Linolenic Acid from Peril a Seed Oil by Urea Inclusion Method

采用响应面分析法对尿素包合法提纯紫苏籽油中α-亚麻酸工艺进行优化。在单因素试验基础上,以α-亚麻酸提纯率为考察指标,考察脂肪酸与尿素溶液体积比、尿素溶液浓度、包合温度及包合时间四个因素对α-亚麻酸提纯率的影响,优化尿素包合法提纯紫苏籽油中α-亚麻酸工艺。结果表明尿素包合法提纯紫苏籽油中α-亚麻酸最佳工艺参数为:混合脂肪酸与尿素溶液体积比1︰50,尿素-乙醇溶液浓度1.0 mol/L,包合温度-20℃,包合时间18.5 h。在此最优条件下紫苏籽油α-亚麻酸提纯率为77.23%。

The α-linolenic acid was purified from perilla seed oil by urea inclusion method with response surface methodology. With α-linolenic acid purification rate as index, based on the single factor test, the effects of volume ratio of mixed fatty acids and urea solution, concentration of urea-ethanol solution, inclusion temperature and inclusion time on α-linolenic acid purification rate was studied. The results showed that the optimal parameters of purification of α-linolenic acid from perilla seed oil by urea inclusion method was as followings： the volume ratio of mixed fatty acids and urea solution was 1︰50, concentration of urea-ethanol solution was 1.0 mol/L, inclusion temperature was-20 ℃, and inclusion time was 18.5 h. Under the optimum conditions, perilla oil α-linolenic acid purification was 77.23%.