细菌培养法、PGR检验法及革兰染色法检验阴道细菌的对比研究

Contrastive study between bacteria culture method and PCR test method and Gram staining method in vaginal bacteria detection

目的：探究与分析细菌培养法、PCR检验法及革兰染色法检验阴道细菌的临床准确性。方法：回顾性分析我院妇科于2013年5月至2015年5月收治的90例患者的临床资料，收集其阴道分泌物分别给予细菌培养法、PCR检验法及革兰染色法行阴道细菌检验，检测的细菌包括加特纳菌、肠球菌、棒状杆菌等。结果：细菌培养法检出率为74．44％，PCR检验法检出率为88．22％，PCR检验法较细菌培养法相比检出率明显升高，具有统计学意义（P〈0．05）。革兰染色检验法检出率为48．89％，细菌培养法法检出率为74．44％，细菌培养法较革兰染色法相比检出率明显升高，具有统计学意义（P〈0．05）。PCR检验法检出率为82．22％，革兰染色法检出率为48．89％，PCR检验法较革兰染色法相比检出率明显升高，具有统计学意义（P〈0．05）。结论：PCR检验法相比于细菌培养法及革兰染色法具有较高的敏感性及特异性，且操作简便，为临床治疗提供可靠的治疗基础，值得推广与应用。

Objective ： To analysis the accuracy of bacterial culture, PCR and the gram staining in the detecion of vaginal bacteria. Methods ： A retrospective analysis of department of gynaecology in May 2013 to May 2015. were cure the clinical data of 90 patients, collect the vaginal secretions were given bacteria culture method, PCR test and gram staining line of vaginal bacteria inspection, detection of bacteria including gartner bacteria, enterococcus, corynebacterium, etc. Results ： The bacter, ial culture method detection rate was 74.44%, the PCR test detection rate was 88.22%, the PCR test is compared bacterial chltuie/nethdd was much higher, with statistical significance （P 〈 0.05）. Gram stain test detection rate was 48.89%, the bacteria culture n^ethod＇method detection rate was 74.44%, the bacteria culture method more. gram staining detection rate increased significantly, compared with a statistical significance （P 〈 0.05）. PCR test detection rate was 82.22%, gram staining detection rate was 48.89%, the PCR test a gram staining detection rate increased significantly, compared with statistical significance （P 〈 0.05）. Conclusions ： PCR test compared to the bacteria culture discipline gram staining method has high sensitivity and specificity, and simple operation, providing reliable treatment basis for clinical treatment, is worthy of popularization and application.