黑果枸杞的组织培养和快速繁殖

Tissue culture and rapid propagation of Lycium ruthenicum Murr

本研究以黑果枸杞(Lycium ruthenicum)为对象,对其愈伤组织诱导、不定芽分化、诱导生根的培养条件进行筛选,并探究了黑果枸杞实验室试管苗的大田移栽.结果表明,诱导愈伤组织最适培养基为:MS+0.5mg·L-1 6-BA+0.1mg·L-1 2,4-D+10g·L-1蔗糖,出愈率达88%,愈伤组织生长状态良好;不定芽分化增殖的最适培养基为:MS+0.2mg·L-1 6-BA+10g·L-1蔗糖,不定芽增殖倍数达5.05倍;诱导生根最适培养基为:MS+0.1mg·L-1 IBA+5g·L-1蔗糖,生根率为95%.逐级炼苗后的试管苗大田移栽成活率达90%.

Tissue culture of Lycium ruthenicum is studied to select the best culture condition including callus inducing,redifferentiation of adventitious buds,rooting.And then,the field transplanting of Lycium ruthenicumtube seedlings preliminary explored.The result shows that the best medium of callus inducing is MS supplemented with 0.5mg·L-1 6-BA,0.1mg·L-1 2,4-D and 10g·L-1 sucrose,the callus inducing rates is 88%.The best medium of differentiation of adventitious buds is MS supplemented with0.2mg·L-1 6-BA and 10g·L-1 sucrose,the proliferation times can be 5.05.The best medium of rooting is MS supplemented with 0.1mg·L-1 IBA and 5g·L-1 sucrose,the rooting rates is 95%.The survive rate of field transplanting is 90%.