番茄红素的抗氧化活性研究

The antioxidant activity studies of lycopene

番茄红素是最主要的类胡萝卜素,且具有多种生理活性.为了促进番茄红素在医药方面的应用,通过铁氰化钾法、脱氧核糖降解法、邻苯三酚自氧化法以及硫代巴比妥酸比色法检测番茄红素的还原力,以及对羟自由基、超氧阴离子的清除作用和对脂质过氧化的抑制作用;用MTT法、SDS-PAGE法以及毛细管电泳检测番茄红素对细胞、蛋白质以及DNA氧化损伤的保护作用,用槲皮素为对照,研究番茄红素的抗氧化活性.结果表明,番茄红素具有一定的还原力和较强的自由基清除能力,对羟自由基和超氧阴离子的EC50分别为0.02和69.31μmol·L-1;抑制脂质过氧化的IC50为20.8μmol·L-1,在浓度为5μmol·L-1时对细胞、DNA的氧化损伤具有明显保护作用,且活性均比槲皮素强.表明番茄红素具有较强抗氧化活性.

Lycopene is one of the most abundant carotenoid which has multiple activities.In order to promote the application of lycopene in medicine,its antioxidant effect is discussed in this papper.The reducing activity,scavenging effects of both hydroxyl radical and superoxide anion,protections against the lipid peroxidation are measured by potassium ferricyanide method,deoxyribose degradation method,autoxidation of pyrogallol method and thiobarbituric acid colouration method,respectively.The detection of and oxidative damage of cells,protein,DNA are done by MTT method,SDS-PAGE,capillary electrophoresis,and all the test are compared with quercetin.The results indicate that lycopene shows some reducing activity and powerful ability of scavenging on free radicals.The EC50 for the elimination of hydroxyl radical and superoxide anion are 0.02 and 69.31μmol·L-1,respectively.Lycopene shows inhibiting effect of lipid peroxidation with IC50 value of 20.8μmol·L-1 and obviously protective effect on cells and DNA from H2O2-induced oxidative damage at the concentration of 5μmol·L-1.Also,lycopene is more active than quercetin in these systems.