太行菊提取物对乙型肝炎病毒的抑制作用

Inhibition of Opisthopappus taihangensis extracts on Hepatitis B virus

目的研究太行菊Opisthopappus taihangensis和野菊花Dendranthema indicum不同部位、不同浓度水提取物对乙型肝炎病毒(HBV)的抑制作用。方法提取并配置不同浓度的太行菊和野菊花的花和茎部位的水提取液,分别以拉米夫定(3TC)和绿茶提取物(GTE)作对照,采用酶联免疫反应检测太行菊花和茎提取物对HBs Ag、HBe Ag的抑制作用,采用实时定量PCR检测不同浓度提取物对HBV复制过程中HBV RNA的影响,荧光素酶报告基因检测不同提取物对HBV转录的影响。结果酶联免疫反应结果表明,1μg/m L太行菊茎提取物能够在作用48、96 h时抑制HBs Ag和HBe Ag,而1μg/m L野菊花茎提取物只能在作用48 h时抑制HBs Ag和HBe Ag;0.1μg/m L太行菊花提取物与1μg/m L野菊花花提取物对HBs Ag和HBe Ag的抑制作用相当。荧光素酶报告基因检测结果说明,太行菊花和茎提取物相对野菊花和茎提取物对HBV启动子质粒CP有抑制作用。PCR结果显示,太行菊花和茎提取物在低浓度时对HBV RNA合成具有一定的抑制作用。结论太行菊水提取物和野菊花提取物都能够不同程度抗HBV,但太行菊效果优于野菊花。

Objective To investigate the inhibitory effect of different parts and different concentration extracts from Opisthopappus taihangensis and Dendranthema indicum on Hepatitis B virus. Methods The solutions of flowers and stems from O. taihangensis and D. indicum with different concentration were extracted and prepared, respectively. Lamivudine（3TC） and green tea extract（GTE） were used as controls. Inhibition of HBsAg and HBeAg were detected using enzyme-linked immune response. The effect of different concentrations of extracts on RNA HBV in HBV replication were detected by real time quantitative PCR. And effect of different extracts on HBV transcription were detected by Luciferase report gene. Results Both HBsAg and HBeAg expression were decreased significantly post-treatment 48 h, 96 h with 1 μg/mL O. taihangensis stem extracts. D. indicum stem extracts（1 μg/mL） could suppress HBsAg and HBeAg at 48 h. The inhibition of 0.1 μg/mL O. taihangensis flower extracts on HBV could reach the effect of 1 μg/mL D. indicum flower extracts. The luciferase reporter gene detection results showed the inhibition of O. taihangensis stem extracts and flower extracts on Luciferase HBV CP. PCR results showed that O. taihangensis stem extracts and flower extracts could inhibit the synthesis of HBV RNA at low concentration. Conclusion Both O. taihangensis and D. indicum extracts have anti-HBV effects. And this anti-viral effect of O. taihangensis is better than that of D. indicum.