利拉鲁肽对高血脂小鼠血脂的影响

Effects of liraglutide on control of blood lipid in hyperlipemia mice

目的研究利拉鲁肽对高血脂小鼠体质量和血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-C)及高密度脂蛋白(HDL-C)的影响。方法将40只小鼠,随机分为对照组、模型组、阳性组及利拉鲁肽低、高剂量组。对照组饲喂基础饲料,其余4组均饲喂高脂饲料。对照组和模型组均ip生理盐水,阳性组ig辛伐他汀,利拉鲁肽低、高剂量组每日分别ip 200、400μg/kg利拉鲁肽1次,连续4周。实验结束处死小鼠,称质量,检测各组小鼠给药前后血清中TC、TG、LDL-C、HDL-C水平,并计算动脉粥样硬化指数(AI)和抗动脉粥样硬化指数(AAI)。结果与对照组相比,模型组小鼠体质量及血脂指标明显升高;与模型组相比,阳性组、利拉鲁肽低、高剂量组小鼠体质量及血脂指标明显降低,且利拉鲁肽的效果明显优于辛伐他汀;与模型组相比,利拉鲁肽低剂量组的AI显著性降低,利拉鲁肽高剂量组的AAI显著升高。结论利拉鲁肽能具有很好的调血脂、抑制动脉粥样硬化的功效,在预防心血管疾病方面具有很好的应用前景。

Objective To study the effect of liraglutide on body weight and total cholesterol（TC）, triglyceride（TG）, low density lipoprotein（LDL-C）, and high density lipoprotein（HDL-C） in serum of hyperlipemia mice. Methods Forty mice were randomly divided into basic group（NFD）, high-fat group（HFD）, simvastatin group（SIM ＋ HFD）, low-dose liraglutide group（LL ＋ HFD）, and high-dose liraglutide group（HL ＋ HFD）. The mice in NFD group were fed with the basal diet, while the mice in other four groups were fed with the high-fat diet. Besides, the mice in both NFD and HFD groups were ip administered with saline, the mice in SIM ＋ HFD group were ig treated with simvastatin daily, the mice in LL ＋ HFD and HL ＋ HFD groups were ip injected with liraglutide（200 and 400 μg/kg）, respectively. Four weeks later, mice were sacrificed and weighed, and the levels of TC, TG, LDL-C and HDL-C in serum were measured, and then atherosclerosis index（AI） and anti-atherosclerosis index（AAI） were further calculated. Results Compared with mice in NFD group, the body weight and levels of serum TC, TG, and LDL-C in mice of HFD group were significantly increased. Compared with mice in HFD group, mice in SIM ＋ HFD, LL ＋ HFD, and HL ＋ HFD groups exhibited much lower body weight and levels of serum TC, TG, LDL-C, and HDL-C. Furthermore, the results also showed that the effect of liraglutide was higher than those of simvastatin, the positive control drug. In addition, compared with mice in HFD group, AI of mice in the LL ＋ HFD group was decreased, and AAI of mice in the LL ＋ HFD group was significantly increased. Conclusion Liraglutide can significantly improve the weight of obese mice fat diet induced obese mice weight gain, dyslipidemia, and the effect is better than that of the positive control drug simvastatin. In addition, liraglutide also have a good effect on prevention of ahterosclerosis.