不同固定液和时间对大鼠睾丸组织病理学制片质量的影响

Effects of different fixatives and fixation time on testis histopathology of rats

目的探讨不同固定液和固定时间对大鼠睾丸组织病理学制片质量的影响。方法选用雄性SD大鼠12只,取两侧睾丸组织,共24例标本。每例标本分别用不同的固定液(10%中性福尔马林溶液、30%Davidson固定液及FAA固定液)固定不同的时间(24、48 h)后,进行脱水、包埋、切片、HE染色。用LEICA DM5000B普通光学显微镜观察。结果 3种固定液固定24 h后,可见30%Davidsons固定液组睾丸组织的异常变化程度低于10%中性福尔马林组和FAA组;固定时间对睾丸组织有一定程度的影响:固定24 h的异常变化程度低于固定48 h。结论 30%Davidson固定液组SD大鼠睾丸组织病理学变化程度最小;固定时间不宜超过24 h。

Objective To investigate the effects of different fixatives and fixation time on testis histopathology of rats. Methods A total of 24 testis tissue samples were collected from 12 male rats. Every specimen was for dehydration, embedding, sectioning, and HE staining after fixing for 24 or 48 h in different fixatives（10% neutral buffered formalin, 30% Davidsons fixative, and FAA fixative）, then the tissues were observed by LEICA DM5000 B ordinary optical microscopy. Results The testis histopathology changes by 30% Davidsons fixative were less than those by 10% neutral buffered formalin and FAA fixative after fixed for 24 h; The fixation time had a certain effect on testis tissue： histopathology change of 24 h was less than that of 48 h. Conclusion Abnormal change of testis histopathology in rats in 30% Davidsons fixative was minimized; Fixation time should not be more than 24 h.