水飞蓟宾联合5-FU对胃癌细胞株MGC803恶性生物学行为的抑制作用研究

Inhibitory effect of silibinin combined with 5-FU treatment on malignant biological behaviors of gastric cancer cell lines MGC803

目的:研究水飞蓟宾联合5-FU处理对胃癌细胞株MGC803恶性生物学行为的抑制作用。方法:培养胃癌细胞株MGC803,分为NC组、5-Fu组、SB+5-Fu组并用不同条件进行处理,检测凋亡细胞数目、侵袭细胞数目以及增殖、侵袭相关基因的表达量。结果:处理后6h、12h、18h、24h时,5-Fu组、SB+5-Fu组凋亡细胞的数目显著多于NC组、侵袭数目显著少于NC组,SB+5-Fu组凋亡细胞的数目显著多于5-Fu组、侵袭数目显著少于5-Fu组(P<0.05);5-Fu组、SB+5-Fu组细胞中Vav3、PTP1B、GOLPH3、RUNX3、Sipa1、UbcH10、NEDD9、Mig-7、CD157、AEP、Galectin-1的mRNA含量低于NC组(P<0.05);SB+5-Fu组细胞中Vav3、PTP1B、GOLPH3、UbcH10、NEDD9、Mig-7、CD157、AEP、Galectin-1的mRNA含量低于5-Fu组(P<0.05),RUNX3、Sipa1的mRNA含量与5-Fu组差异无统计学意义(P>0.05)。结论:与5-FU单药处理相比,水飞蓟宾联合5-FU处理能够更为有效的促进胃癌细胞凋亡、抑制胃癌细胞侵袭、调节增殖和侵袭相关基因的表达。

Objective：To study the inhibitory effect of silibinin combined with 5-FU treatment on malignant biological behaviors of gastric cancer cell lines MGC803.Methods：Gastric cancer cell lines MGC803 were cultured,divided into NC group,5-Fu group and SB＋5-Fu group and treated with different conditions,and then the number of apoptotic cells,the number of invasive cells as well as the expression of proliferation and invasion-related genes were detected.Results：6h,12 h,18h and 24 hafter treatment,the number of apoptotic cells of 5-Fu group and SB＋5-Fu group was significantly more than that of NC group,the number of invasive cells was significantly less than that of NC group,the number of apoptotic cells of SB＋5-Fu group was significantly more than that of 5-Fu group,and the number of invasive cells was significantly less than that of 5-Fu group;mRNA contents of Vav3,PTP1 B,GOLPH3,RUNX3,Sipa1,UbcH10,NEDD9,Mig-7,CD157,AEP and Galectin-1of 5-Fu group and SB＋5-Fu group were lower than those of NC group;mRNA contents of Vav3,PTP1 B,GOLPH3,UbcH10,NEDD9,Mig-7,CD157,AEP and Galectin-1of SB＋5-Fu group were lower than those of 5-Fu group,and mRNA contents of RUNX3 and Sipa1were not different from those of 5-Fu group.Conclusion：Compared with single 5-FU treatment,silibinin combined with 5-FU treatment can more effectively promote gastric cancer cell apoptosis,inhibit gastric cancer cell invasion and regulate the expression of proliferation and invasion-related genes.