鼠神经生长因子对早产儿脑发育影响的研究

Effect of mouse nerve growth factor on brain development in premature infants

目的：分析新生儿期应用鼠神经生长因子对早产儿脑发育的影响。方法：选取2015年1月1日~2015年12月30日间在佛山市南海区人民医院分娩的早产儿共37例作为研究对象,根据干预方式不同分为观察组（n=18）及对照组（n=19）。对照组患儿不给予外源性药物,观察组患儿在新生儿期接受鼠神经生长因子（NGF）治疗,对比两组患儿的干预后脑部核磁共振、脑电图、脑干听觉诱发电位检查结果,盖泽尔发育量表评分值,NSE、S-100β、8-OHdG、8-I-PGF2α水平,TLR-4、TNF-α、IL-18等水平差异。结果：观察组患儿的MRI、EEG、BAEP检查正常比例均高于对照组患儿,重度异常比例显著低于对照组患儿（均P〈0.05）;观察组患儿的纠正胎龄3月及6月的运动、适应性行为、语言、社交能力等盖泽尔发育量表评分值均高于对照组患儿（均P〈0.05）;观察组患儿纠正胎龄后3月、6月的血清NSE、S-100β、8-OHdG、8-I-PGF2α水平均低于对照组患儿（均P〈0.05）;观察组患儿纠正胎龄后6月的血清TLR-4、TNF-α、IL-18、NF-κB、MMP-9水平低于对照组患儿,EGF、SOD水平高于对照组患儿（均P〈0.05）。结论：早产儿新生儿期应用鼠神经生长因子,有助于促进神经细胞生长发育、优化早产儿脑功能,具有积极的临床意义。

Objective：To analyze the effect of application of mouse nerve growth factor in neonatal period on brain development in premature infants.Methods：A total of 37 premature infants given birth in our hospital from 1st January,2015to30 th December,2015 were selected as research subjects and divided into observation group（n=18）and control group（n=19）according to different ways of intervention.Control group didn＇t receive exogenous drugs,observation group received mouse nerve growth factor（NGF）treatment in neonatal period,and then differences in brain magnetic resonance imaging,electroencephalogram,brainstem auditory evoked potential,scores of Gesell developmental scale,levels of NSE,S-100β,8-OHdG and8-I-PGF2αand levels of TLR-4,TNF-α,IL-18 and so on of two groups after intervention were compared.Results：Proportions of normal MRI,EEG and BAEP of observation group were higher than those of control group,and proportions of severely abnormal were significantly lower than those of control group（P〈0.05）;scores of Gesell developmental scale motor,adaptive behavior,language and social skills of observation group in 3 months and 6 months of corrected gestational age were higher than those of control group（P〈0.05）;serum NSE,S-100β,8-OHdG and 8-I-PGF2αlevels of observation group after 3months and 6months of corrected gestational age were lower than those of control group（P〈0.05）;serum TLR-4,TNF-α,IL-18,NF-κB and MMP-9levels of observation group after 6months of corrected gestational age were lower than those of control group,and levels of EGF and SOD were higher than those of control group（P〈0.05）.Conclusion：Application of mouse nerve growth factor in neonatal period of premature infants helps to promote nerve cell growth and development and optimize brain function of premature infants,and it has active clinical significance.