红腹锦鸡胚胎期羽毛芽发育的研究

Development Characteristics of Feather Bud of Chrysolophus pictus in Embryonic Period

旨在了解红腹锦鸡胚胎期羽毛毛囊的组织结构及毛囊形态发生的变化过程,为研究红腹锦鸡羽毛毛囊发育的分子调控机理奠定组织学基础。分别对5、7、8、9、10、11、13、16、18日胚龄的红腹锦鸡胚胎发育情况进行实体观察;采集上述胚龄的红腹锦鸡背部皮肤,制作石蜡切片并进行HE染色,在显微镜下观察毛囊形态结构并拍照记录。红腹锦鸡胚胎于9日胚龄时羽毛芽开始发育,可以观察到色素在毛囊内的沉积,16日胚龄时毛囊发育基本完成,体表覆盖羽毛。该研究为进一步阐明红腹锦鸡羽毛发育的分子机制奠定了组织学基础。

The aims of the present study were to observe the histological structure of feather follicles of Chrysolophus pictus in embryonic period and its morphological changes, and to provide a histological base for the investigation of molecular regulation mechanism of Chrysolophus pictus feather follicles development. The development characteristics of Chrysolophus pictus embryos with 5, 7, 8, 9, 10, 11, 13, 16, 18-day embryonic ages were observed, respectively. The back skin of Chrysolophus pictus embryos with above-mentioned embryonic ages were collected respectively and were used to prepare paraffin sections which were subsequently stained using a standard HE staining method. The morphological structure of feather follicles was observed using an optical microscope. The feather buds development of Chrysolophus pictus embryo with 9-day embryonic age was initially observed, and the pigment deposition in feather follicles was found. In 16-day embryonic age, the development of feather follicles was basically completed, and the body surface of Chrysolophus pictus embryo was covered with feather. The results of the study may provide a histological base for clarifying the molecular regulation mechanism of feather development of Chrysolophus pictus.