摘要
采用金黄色葡萄球菌肠毒素A（SEA）和金黄色葡萄球菌肠毒素B（SEB）免疫Balb/c小鼠制备相应的单克隆抗体（m Ab）,并以此为基础建立SEA和SEB的ELISA检测方法,用于牛乳中金黄色葡萄球菌肠毒素的检测。SEA双抗体夹心法ELISA的曲线范围是1~16μg/L,相关系数R2=0.9996,检测牛乳的检测限是0.431μg/L,加标4μg/L和10μg/L,准确度分别为89.18%和97.62%。SEB双抗体夹心法ELISA的曲线范围是0.5~8μg/L,相关系数R2=0.9998,检测牛乳的检测限是0.335μg/L,加标4μg/L和10μg/L,准确度分别为107.83%和89.88%。
Staphylococcal enterotoxin A B were adopted to immune Balb/c mouse. A two-site monoclonal antibody quantitative enzyme-linked immunosorbent for detecting SEA and SEB were established in this study. The detection limit of SEA established in this study was 0.431μg/L with a detection concentration range of 1~16μg/L and correlation coefficient R2=0.9996. The recovery of artificially contaminated sample were 89.18% and 97.62% respectively. The detection limit of SEB established in this study was 0.335μg/L with a detection concentration range of 0.5~8μg/L and correlation coefficient R2=0.9998. The recovery of artificially contaminated sample were 107.83% and 89.88% respectively.
出处
《中国奶牛》
2016年第3期32-35,共4页
China Dairy Cattle
基金
首都市民健康培育项目(课题编号:Z131100004013014)
