饲粮L-精氨酸含量对冬毛期雌性水貂氨基酸消化率、血清氨基酸含量及血清生化指标的影响

Effects of Dietary L-Arginine Content on Amino Acid Digestibility,Serum Amino Acid Contents and Serum Biochemical Indices of Female Mink in the Winter Fur-Growing Period

本试验旨在研究饲粮L-精氨酸含量对冬毛期雌性水貂氨基酸消化率、血清氨基酸含量及血清生化指标的影响。选取(150±3)日龄的健康雌性水貂63只,随机分为7组(每组9个重复,每个重复1只水貂),分别饲喂在基础饲粮中添加0、0.20%、0.40%、0.60%、0.80%、1.00%和1.20%L-精氨酸的试验饲粮,试验饲粮中L-精氨酸含量分别为1.70%、1.90%、2.10%、2.30%、2.50%、2.70%和2.90%。预试期7 d,正试期60 d。结果表明:1)干物质采食量、干物质排出量和干物质消化率各组之间差异不显著(P>0.05)。2)1.70%组水貂的赖氨酸消化率显著高于2.50%、2.70%和2.90%组(P<0.05)。2.70%和2.90%组水貂的精氨酸消化率显著高于1.70%和1.90%组(P<0.05)。3)2.30%组水貂血清丝氨酸和脯氨酸含量显著高于1.70%、1.90%、2.10%和2.90%组(P<0.05)。2.30%和2.50%组水貂血清丙氨酸含量显著高于1.70%、1.90%、2.10%和2.90%组(P<0.05)。2.30%组水貂血清苯丙氨酸含量显著高于1.70%和2.90%组(P<0.05)。2.30%组水貂血清精氨酸含量显著高于1.70%、1.90%、2.10%和2.90%组(P<0.05)。4)2.30%组水貂血清天门冬氨酸氨基转移酶活性显著高于1.70%组(P<0.05)。2.30%和2.50%组水貂血清乳酸脱氢酶活性显著高于1.70%、1.90%、2.10%、2.70%和2.90%组(P<0.05)。5)2.30%和2.50%组水貂血清免疫球蛋白M含量显著高于1.70%、1.90%、2.10%、2.70%和2.90%组(P<0.05)。2.30%组水貂血清免疫球蛋白G含量显著高于1.70%和2.90%组(P<0.05)。2.30%和2.50%组水貂血清补体4含量显著高于1.70%和2.90%组(P<0.05),2.10%组水貂血清补体4含量显著高于1.70%(P<0.05)。由结果可知,当饲粮中L-精氨酸含量为2.30%时,冬毛期雌性水貂能获得较好的蛋白质和氨基酸代谢,同时免疫功能得到增强。

This study was conducted to study the effects of dietary L-arginine content on amino acid digestibility,serum amino acid contents and serum biochemical indices of female mink in the winter fur-growing period. Sixty-three healthy female mink at the age of（ 150 ± 3） days were randomly divided into 7 groups with 9 replicates each and 1 mink per replicate. The mink in the seven groups were fed experimental diets with L-arginine supplemental levels of 0,0. 20%,0. 40%,0. 60%,0. 80%,1. 00% and 1. 20%,and the L-arginine content in the experimental diets were 1. 70%,1. 90%,2. 10%,2. 30%,2. 50%,2. 70% and 2. 90%,respectively. The pre-test period lasted for 7 days,and the trial lasted for 60 days. The results showed as follows： 1） the dry matter（ DM） intake,DMoutput and DMdigestibility had no significant difference among all groups（ P〈 0. 05）.2） The digestibility of lysine in 1. 70% group were significantly higher than those in 2. 50%,2. 70% and 2.90% groups（ P〈 0. 05）. The digestibility of arginine in 2. 70% and 2. 90% groups was significantly higher than that in 1. 70% and 1. 90% groups（ P 〈0. 05）. 3） The contents of serum proline and serine in 2. 30% group was significantly higher than that in 1. 70%,1. 90%,2. 10% and 2. 90% groups（ P 〈0. 05）. The content of serum alanine in 2. 30% and 2. 50% groups was significantly higher than that in 1. 70%,1. 90%,2. 10% and2. 90% groups（ P〈 0. 05）. The content of serum phenylalanine in 2. 30% group was significantly higher than that in 1. 70% and 2. 90% groups（ P〈 0. 05）. The content of serum arginine in 2. 30% group was significantly higher than that in 1. 70%,1. 90%,2. 10% and 2. 90% groups（ P〈 0. 05）. 4） The activity of serum aspartate amino transferase in 2. 30% group was significantly higher than that in 1. 70% group（ P〈 0. 05）. The activity of serum lactate dehydrogenase in 2. 30% and 2. 50% groups was significantly higher than that in 1. 70%,1.90%,2. 10%,2. 70% and 2. 90% groups（ P 〈0. 05）. 5） The content of serum immunoglobulin Min 2. 30%and 2. 50% groups was significantly higher than that in 1. 70%,1. 90%,2. 10%,2. 70% and 2. 90% groups（ P〈 0. 05）. The content of serum immunoglobulin G in 2. 30% group was significantly higher than that in 1.70% and 2. 90% groups（ P〈 0. 05）. The content of serum complement 4 in 2. 30% and 2. 50% groups was significantly higher than that in 1. 70% and 2. 90% groups（ P〈 0. 05）,and the content of serum complement 4in 2. 10% group was significantly higher than that in 1. 70% group（ P〈 0. 05）. So,when the dietary L-arginine content is 2. 30%,the female mink in the winter fur-growing period can obtain better protein and amino acid metabolism,and enhance the immune function.