骨关节炎患者血清中炎性因子IL-1β、IL-6和COX-2的表达

Serum Expression of Inflammatory Cytokines IL-1β,IL-6 and COX-2 in Osteoarthritis Patients

目的:探讨骨关节炎患者血清中炎性因子白细胞介素-1β(IL-1β)、IL-6、环氧合酶-2(COX-2)的表达。方法:选择在上海市光华中西医结合医院痛风科就诊的骨关节炎患者72例,分为发病组36例、治愈组36例,以同期门诊就诊的无骨关节炎及其他系统严重疾病的健康人20例作为对照组。各组分别采血、分离血清,用酶联免疫吸附法测定患者血清中炎性因子IL-1β、IL-6、COX-2的表达情况。结果:骨关节炎患者中,炎性因子IL-1β、IL-6、COX-2呈现高表达趋势;发病组IL-1β、IL-6、COX-2的表达量高于对照组和治愈组(P<0.01)。结论:炎性因子在骨关节炎患者中高表达,治愈后表达量下降;IL-1β、IL-6、COX-2可作为骨关节的临床诊断和治疗指标。

Objective：To explore the serum expression of inflammatory cytokines interleukin-1β（IL-1β）,interleukin-6（IL-6）and cyclooxygenase-2（COX-2）in patients with osteoarthritis.Methods：Seventy-two patients with osteoarthritis,who were admitted to Gout Department of Shanghai Guanghua Hospital,were selected and divided into disease group and cure group.In the same period,twenty healthy individuals,who had no osteoarthritis and severe disease in other systems,were set as control group.Blood sampling and serum separation were conducted in each group.And the serum expression of inflammatory cytokines IL-1β,IL-6,and COX-2 in osteoarthritis patients was determined by enzyme linked immunosorbent assay.Results：In osteoarthritis patients,inflammatory cytokines IL-1β,IL-6 and COX-2 presented a tendency of high expression.The expression levels of IL-1β,IL-6 and COX-2 in diseased group were higher than those in control group and cure group（P〈0.01）.Conclusions：The inflammatory cytokines present high expression in patients with osteoarthritis and the expression decrease after cure.IL-1β,IL-6 and COX-2 can be used as clinical diagnostic and therapeutic index of osteoarthritis.