免疫荧光蛋白质芯片法检测血清特异性IgE 1200例分析

Detection of specific IgE by immunofluorescence protein chip in 1200 patients

目的:比较免疫荧光蛋白质芯片法与目前临床应用的免疫印迹法检测特异性IgE的敏感性和特异性。方法:分别用免疫荧光蛋白质芯片试剂盒和免疫印迹试剂盒检测1200例过敏性皮肤病患者血清中6种过敏原(尘螨、猫毛皮屑、鸡蛋清、虾、蚌与蟹)特异性IgE抗体。结果:免疫荧光蛋白质芯片法检测的阳性率为:尘螨62.5%、猫毛皮屑25.3%、鸡蛋清34.7%、虾36.4%、蟹41.5%;免疫印迹法阳性率分别为:尘螨60.2%、猫毛皮屑23.3%、鸡蛋清26.6%、虾33.8%、蟹36.7%。此5种过敏原用两种方法检测结果具有良好一致性;免疫荧光蛋白质芯片检测出蚌特异性IgE阳性率为27.6%,免疫印迹法仅为0.3%,用蚌标准阳性血清证实免疫荧光蛋白质芯片法结果可靠。结论:免疫荧光蛋白质芯片试剂盒与免疫印迹诊断试剂盒的检测结果具有良好的一致性,几种方法对于蚌特异性IgE检测结果的不一致性可能与过敏原来源有关。免疫荧光蛋白质芯片法检测过敏原特异性IgE敏感、特异、快速。

Objective：To determine the allergens is important for the diagnosis and treatment of allergic dermatoses.In this study,we used a novel immunofluorescence protein chip method to detect allergen-specific Ig E,and compared the sensitivity and specificity of protein chip method with a widely used immunoblot method.Methods：Protein chip and immunoblot method were used to detect six allergen-specific Ig E in sera from 1200 patients with allergic skin diseases.Results：Using chip method,the positive rates for specific Ig E were 62.5%（house dust mite）,25.3%（cat dander）,34.7%（egg white）,36.4%（shrimp）,and 41.5%（crab）.Similarly,immunoblot method showed 60.2% positive for house dust mite,23.3% for cat dander,26.6% for egg white,33.8% for shrimp,and 36.7% for crab.However,positive rates for clam were different between these two methods（immunoblot,0.3%;protein chip,27.6%）.The positive rate for clam was validated using positive control,clam-specific Ig E serum.Conclusions：The protein chip method showed high consistency with immunoblot method for five allergens.The difference for the clam-specific Ig E might be due to the difference of allergen source.The results of the present study suggest that the protein chip assay is highly sensitive.