CD177＋中性粒细胞与炎症性肠病肠黏膜损伤关系的研究

CD177＋ neutrophils and its correlation with mucosal impairment in patients with inflammatory bowel disease

目的通过对比炎症性肠病患者外周血CD177＋中性粒细胞的比例及肠黏膜炎症部位CD177＋中性粒细胞的浸润与正常对照者的差异，探讨CD177＋中性粒细胞与肠道黏膜损伤及临床特征的相关性。方法研究对象为溃疡性结肠炎患者54例、克罗恩病患者62例和正常对照者48例。对炎症性肠病患者和正常对照者，分别应用SYBR-green Reahime PCR等方法，分析患者外周血及肠黏膜炎症部位CD177＋中性粒细胞的比例，研究CD177＋中性粒细胞与内镜下疾病活动性及临床疾病活动程度的关系。结果炎症性肠病患者外周血和炎症部位肠黏膜组织中，CD177的表达均显著高于正常对照者及缓解期患者（P〈0．05），CD177＋中性粒细胞的比例显著增加（P〈0．05）。炎症性肠病患者外周血中性粒细胞和炎症部位肠黏膜组织中CD177的表达与内镜下疾病活动性及临床疾病活动程度均呈明显正相关（P〈0．05）。结论在炎症性肠病患者外周血及炎症部位肠黏膜组织中CD177＋中性粒细胞的比例增加，能够反映内镜下疾病活动程度和临床疾病活动程度。CD177＋中性粒细胞可能作为中性粒细胞的一个重要亚群参与肠道的免疫病理过程。

Objective To investigate the percentage of CD177＋ neutrophils in peripheral blood and inflamed mucosa of the intestine in patients with inflammatory bowel disease （IBD） and in healthy controls, and to investigate the correlation between CD177＋ neutrophils and mucosal impairment as well as clinical characteristics. Methods A total of 54 patients with ulcerative colitis （ UC）, 62 patients with Crohn disease （CD） , and 48 healthy controls were enroUed. SYBR-green Realtime PCR was applied to determine the ex- pression proportion of CD177＋ neutrophils in peripheral blood and in inflamed intestinal mucosa to investigate the correlation between CD177＋ neutrophils and endoscopic and clinical disease activity. Results In periph- eral blood and inflamed mucosa of the intestine, the expression of CD177＋ neutrophils was significantly higher than that in hea[th controls or patients in remission stage （ P 〈 O. 05 ） , with higher proportion （ P 〈 O. 05）. The expression of CD177＋ neutrophils in peripheral blood and inflamed mucosa of the intestine was positively correlated with both endoscopic disease activity index and clinical severity of the disease （P 〈 0.05）. Conclusion CD177＋ neutrophils increase in peripheral blood and intestinal mucosa of patients with IBD. The percentage of CD177＋ neutrophils is a favorable marker for both endoscopic disease activity and clinical severity of the disease. CD177＋ neutrophils may play a vital role in the immune response in the intes- tinal mucosa, as a new subset of effector neutrophils.