不同频率微振动对血管内皮细胞生长因子表达及通透性的影响

Effects of different frequency microvibrations in the vascular endothelial growth factor expression and permeability of vascular endothelial cell

目的研究微振动对血管内皮细胞分泌血管内皮细胞生长因子（VEGF）及通透性的影响。方法培养人脐静脉血管内皮细胞（HUVEC）,每日施加0.2、0.5、2、5 Hz 4种低频率微振动30 min,通过Tagman探针实时荧光定量聚合酶链反应（PCR）及蛋白质印迹法检测VEGF的表达,并检测内皮细胞的通透性。结果加载0.2 Hz和0.5 Hz微振动可使血管内皮细胞VEGF m RNA及蛋白表达上调（P〈0.05）,加载2 Hz和5 Hz振动后VEGF m RNA及蛋白表达下调（P〈0.01）;加载0.2 Hz和0.5 Hz微振动可使血管内皮细胞通透性增强（P〈0.01）,2 Hz和5 Hz微振动则使内皮细胞通透性降低（P〈0.01）。结论 0.2~0.5 Hz的微振动可使血管内皮细胞分泌VEGF增高,并提高血管内皮细胞的通透性。

Objective This study aimed to evaluate the vascular endothelial growth factor（VEGF） expression and permeability of vascular endothelial cell under microvibration. Methods Human umbilical vein endothelial cell（HUVEC） were cultured, randomly vibrated under low frequency of 0.2, 0.5, 2, 5 Hz, 30 min per day. The VEGF m RNA level was detected by Tagman probe real-time fluorescence quantitative polymerase chain reaction（PCR）, and the VEGF protein expression level was detected by Western blot. The permeability of vascular endothelial cell was evaluated. Results Compared with the blank control group, the m RNA and protein expression level of VEGF were significantly increased under 0.2, 0.5 Hz microvibration（P〈0.05）, and decreased under 2, 5 Hz microvibration（P〈0.01）. The vascular endothelial permeability increased under 0.2, 0.5 Hz microvibration（P〈0.01）, whereas the permeability decreased under 2, 5 Hz microvibration（P〈0.01）. Conclusion 0.2-0.5 Hz microvibration can up-regulate the expression of VEGF m RNA and protein in vascular endothelial, and increase the permeability.