离子色谱法测定药物中氨丁三醇的含量

Determination of the content of trometamol in drugs by ion chromatography

目的:建立离子色谱法测定氨丁三醇的含量。方法:采用抑制型离子色谱法,DIONEX Ion Pac CS16(250 mm×5 mm,5.5μm)阳离子交换柱,以水-0.05 mol·L^(-1)甲烷磺酸-50%乙腈(60∶20∶20)为淋洗液,电导检测器。结果:氨丁三醇与钠离子、钙离子分离良好,在20~200μg·m L^(-1)范围内呈良好的线性关系(r=0.999);定量限为5μg·m L^(-1);添加右旋酮洛芬氨丁三醇回收率为100.4%(n=9,RSD为2.8%);添加碘佛醇注射液回收率为99.1%(n=9,RSD为2.3%)。结论:该方法灵敏、准确、简便,专属性好,可用于测定多种药物中氨丁三醇的含量。

Objective： To establish a method to determine the content of trometamol in drugs by ion chromatography. Methods： DIONEX Ion Pac CS16（ 250 mm × 5 mm,5. 5 μm）,water-0. 05 mol·L-1methanesulfonic acid-50% acetonitrile（ 60∶ 20∶ 20）,and conductivity detector were used. Results： Good separation of trometamol,Na＋ and Ca2＋ was achieved. The standard curve was linear in the range of 20 200 μg·m L-1（ r = 0. 999）. The limit of quantification was 5 μg·m L-1. The average recovery was 100. 4%（ adding dexketoprofen trometamol,n =9,RSD = 2. 8%）,or 99. 1%（ adding ioversol injection,n = 9,RSD = 2. 3%）. Conclusion： This method is accurate,simple,and selective. It will be used for the determination of the content of trometamol in various drugs.