绿色化学方法合成儿茶酚-壳聚糖水凝胶的应用

A Green and Mix-to-use Approach for Mussel Inspired Chitasan-Catechol Hydrogel

水凝胶在生物医学领域有重要应用价值,如促进伤口愈合等.利用壳聚糖与2,4,5-三羟基苯甲醛或2,3,4-三羟基苯甲醛通过一步环境友好、副产物为水的生成希夫(Schiff)碱的反应,得到一类壳聚糖-儿茶酚类的水凝胶应用于伤口处理.2,4,5-三羟基苯甲醛修饰的壳聚糖水凝胶(CC-1)能有效抑制金黄色葡萄球菌生长,而2,3,4-三羟基苯甲醛修饰的壳聚糖水凝胶(CC-2)具有极强的黏附性.在小鼠模型上,CC-1促进开放性伤口愈合的效果与商品化药膏(DuoDERM)相当.与文献报道的以1-(3-二甲氨基丙基)-3-乙基二亚胺(EDC)缩合法合成的水凝胶相比,本研究在制备CC-1或CC-2的过程中不需要加入额外的化学试剂,终产物只有水凝胶和水,无需纯化,安全无毒,即配即用.

Hydrogels have important applications in biomedical fields,such as promoting wound healing.Herein we tried to develop chitosan based adhesive hydrogels as tissue adhesives.We developed a green and mix-to-use approach for generation of mussel inspired chitasan-catechol hydrogel.An in situ gel-forming system composed of 2,4,5-three hydroxy benzaldehyde or 2,3,4-three hydroxy benzaldehyde conjugated chitosan derivatives was easily prepared,producing the hydrogels CC-1and CC-2respectively.In vitro study demonstrated that CC-1could inhibit the growth of Staphylococcus aureus whereas CC-2was a superior adhesive.In vivo wound healing study was performed by smearing hydrogels on rat dorsal wounds with a diameter of 10 mm and keeping them on for14 days.Histological results demonstrated that CC-1hydrogel was of high performance and comparable to commercialized wound dressing（DuoDERM）on wound healing.This approach is advantageous as no toxic byproducts are generated during making CC-1and CC-2hydrogels and only water is produced following formation of Schiff-base promoted hydrogels.The mix-to-use technique is of significance for commercial application.