米诺环素对神经病理性疼痛大鼠脊髓神经胶质细胞GTs表达的影响

The effect of minocycline on GTs expression in spinal cord of rats with neuropathic pain

目的:观察米诺环素对神经病理性大鼠脊髓神经胶质细胞的水平谷氨酸转运蛋白(GTs)表达的影响,探讨神经病理性疼痛中的作用机制和治疗方法。方法:将40只成年雄性SD大鼠,采用坐骨神经结扎术建立神经病理性疼痛模型。根据手术类型及注射药物,随机分成4组:A组,假手术组(n=10);B组,手术组(n=10);C组,生理盐水+手术组(n=10);D组,米诺环素+手术组(n=10)。在术后,第1、3、7、11天观察热缩足反射潜伏期(TWL)、测定大鼠机械缩足阈值(MWT)的变化,应用Western Blot方法检测检测大鼠脊髓神经胶质细胞GTs表达。结果:手术组(BCD组)大鼠较假手术组(A组),热缩足反射潜伏期明显缩短,且大鼠50%机械缩足阈值明显降低。鞘内注射米诺环素组(D组)大鼠与对照组(BC组)比较,其热缩足反射潜伏期明显延长,且大鼠50%机械缩足阈值明显升高,并且脊髓神经胶质细胞GTs的表达明显高于BC组。结论:米诺环素能够通过维持大鼠脊髓神经胶质细胞谷氨酸转运蛋白(GTs)表达的影响,从而缓解神经病理性疼痛。

Objective： To analyze the effects of minocycline on glutamate transporters（ GTs） expression in spinal cord of rats during neuropathic pain; and to study the mechanism of neuropathic pain. Methods： Forty adult male Sprague- Dawley（ SD） rats were randomly divided into 4 groups（ n = 10 each） ： Group A,Sham operation group; Group B,operation group; Group C physiological saline and operation group; group D,minocycline and operation group. Thermal withdrawal latency（ TWL） and mechanical withdrawal threshold（ MWT） were measured on1 st,3rd,7th and 11 th days. GTs expression in the dorsal horn was detected by Western blotting. Results： Mechanical tenderness was occurred in Group B,C and D on he 7th day after operation,and then persisted to the end of the experiment. TWL and MWT were decreased significantly in Group B,C,D than those in group A. Compared with Group B and C,Group A was increasing significantly in TWL and MWT. The expression of GTs was higher in Group D than that in group B and C. Conclusion： Minocycline can prevent the development of neuropathic pain,and up- regulate GTs during the development of neuropathic pain.