摘要
目的观察阿特拉津(ATR)处理后小鼠心脏组织中过氧化产物的含量及Nrf2信号通路的活化,探讨ATR导致的心脏组织氧化应激损伤及保护机制。方法以玉米油溶解ATR,将32只雌性BALB/C小鼠随机分为0mg/kg、5mg/kg、25mg/kg和125mg/kg ATR剂量组,灌胃处理28d,于第29d处死小鼠取心脏组织匀浆并提取总蛋白,生化和Western blot方法检测心肌组织中过氧化产物含量、Nrf2信号通路相关蛋白的表达及抗氧化酶活性。结果ATR暴露小鼠心肌组织中NO、MDA含量增高;Nrf2及Keap1表达降低;Ⅱ相解毒酶表达增高;SOD、CAT、GSH-PX含量增加。结论 ATR暴露可诱导小鼠心脏组织活性氧生成增多,Nrf2/ARE通路激活,通过上调Ⅱ相解毒酶及抗氧化酶进行抗氧化防御。
Objective To observe the contents of peroxidation product and the activation of Nrf2 signaling pathway in heart tissue of mouses treated by atrazine（ATR）,and to evaluate the mechanism of ATR on heart tissue oxidative stress injury and its protection mechanism.Methods 32 female BALB/C rats were randomly divided into 4groups,treated by a daily gavage of 0mg/kg（control group）and 5,25 and 125mg/kg（experiment groups）atrazine dissolved in maize oil respectively for 28 days,and the animals were sacrificed on day 29.The heart tissues were collected to homogenize and isolate the total protein.Biochemical process and Western blot were employed to detect the contents of peroxidation product,the expressions of Nrf2 signaling pathways related proteins and the activation of antioxidase in the heart tissues.Results After treated by ATR,the content of NO and MDA were increased;Nrf2and Keap1 were decreased;Ⅱ phase detoxifying enzymes was increased;SOD,CAT,GSH-PX were increased.Conclusion After treated by ATR,the reactive oxygen in heart tissue was increased,and the Nrf2/ARE signaling pathway was activated to resist the oxidation through up-regulating the expression ofⅡ phase detoxifying enzymes and antioxidase
出处
《中国实验诊断学》
2016年第3期355-358,共4页
Chinese Journal of Laboratory Diagnosis
