球状C1q受体在宫颈癌中的表达及其生物学意义

Expression of gC1qR in cervical cancer and its effects on the biological behaviors of human cervical cancer cells

目的球状C1q受体(g C1qR)是一种高度酸性的受体蛋白,除红细胞外几乎所有的哺乳动物细胞都存在g C1qR的表达,可介导多种生物反应。文中探讨g C1qR在宫颈癌中的表达及其生物学意义。方法回顾性分析2014年8月至2015年4月在南京市妇幼保健院妇科手术切除的100例宫颈组织,其中宫颈癌组织与慢性宫颈炎症组织各50例。以宫颈癌细胞系C33a为研究对象,应用免疫组织化学S-P法检测g C1qR蛋白在宫颈组织中的表达及定位情况,采用RT-PCR及Western blot分别检测g C1qR mRNA及蛋白质的表达水平;采用细胞伤痕愈合实验、Transwell实验及流式细胞技术分别对宫颈癌细胞的迁移能力、侵袭能力以及细胞凋亡的情况进行评估。结果 g C1qR mRNA及蛋白质的表达在宫颈癌组织中,表达分别为2.18±0.37和0.27±0.09,均明显低于其配对的宫颈炎症组织7.23±0.69和0.74±0.02,差异有统计学意义(P<0.001);过表达g C1qR基因可显著降低宫颈癌细胞的迁移率[(42.60±3.29)%vs(141.83±4.71)%]及侵袭率[(26.20±2.89)%vs(67.13±0.95)%],细胞凋亡率明显增加[(22.89±1.46)%vs(12.98±0.57)%],且电镜下呈现细胞凋亡的典型特征。结论 g C1qR基因的表达与抑制宫颈癌细胞侵袭与迁移的能力、诱导宫颈癌细胞凋亡存在一定关系,可为宫颈癌的防治提供新线索和潜在的干预靶标。

Objective Globular C1 q receptor（ g C1qR）,a highly acidic receptor protein,is expressed in almost all mammalian cells in addition to exoerythrocytic,which can mediate a variety of biological responses. The study aimed to explore g C1 qR expression in cervical cancer and itd effects on the biological behaviors of human cervical cancer cells. Methods Retrospective analysis was made on 100 cervical tissue samples of patients in Nanjing Maternal and Child Health Hospital from August 2014 to April 2015,including 50 cervicitis tissues and 50 cervical cancer tissues. Immunohistochemical SP method was applied in the research of cervical cancer cell line C33 a to detect the expression and the location of g C1 qR in cervical tissues. Real-time PCR and Western blot analysis were respectively applied to detect the levels of g C1 qR mRNA and g C1 qR protein expression. Besides,the abilities of C33 a cells migration,invasion and apoptosis were respectively assessed by in vitro cell wound healing experiment,transwell assay and flow cytometry. Results The expression of g C1 qR gene was dramatically decreased in the group of cervical cancer tissues when compared with chronic cervictis group（ 2. 18 ± 0. 37 vs 7. 23 ± 0. 69,0. 27 ± 0. 09 vs0. 74 ± 0. 02,P 0. 001）. g C1 qR overexpression could result in significant up-regulation of cervical celluer apoptosis（ [22. 89 ± 1. 46]%vs [12. 98 ± 0. 57]%） and down-regulation in migration（ [42. 60 ±3. 29]% vs [141. 83 ± 4. 71]%） and invasion（ [26. 20 ± 2. 89]%vs [67. 13 ± 0. 95]%）. Typical apoptosis was also observed in cervical cells by transmission electron microscope. Conclusion g C1 qR expression might play an important role in inhibiting the invasion and migration of cervical cancer cells and inducing the apoptosis of cervical carcinoma cells,which provides new clues and potential targets for the treatment of cervical cancer in further research.